Family C108


Summary Holotypes Alignment Tree Genomes Structure Literature

Summary for family C108

Family type peptidaseC108.001 - Prp peptidase (Staphylococcus aureus) (Staphylococcus aureus), MEROPS Accession MER0003820 (peptidase unit: 1-106)
Content of familyFamily C108 contains cysteine endopeptidases
History Identifier created: MEROPS 10.0 (14 Mar 2016)
The Prp protein (C108.001) was discovered to be the peptidase responsible for post-translational trimming of ribosomal protein L27 from (Staphylococcus aureus), removing an N-terminal extension that is absent in most Gram-positive bacteria (Wall et al., 2014). The Prp peptidase is homologous to the orf13 protein from bacteriophage Cp-1 (C108.002), which processes the major capsid protein of the phage (Martin et al., 1998).
Catalytic typeCysteine
Active site residuesH22 C34 
Active siteA His, Cys catalytic dyad has been proposed and mutagenesis of the cysteine prevents cleavage of ribosomal protein L27 by the Prp peptidase (Wall et al., 2014).
Activities and specificitiesThe Prp protein removes a nine-residue extension from the N-terminus of ribosomal protein L27 (Wall et al., 2014). The processing peptidase from bacteriophage Cp-1 removes a 48-residue N-terminal propeptide from the main coat protein (Martin et al., 1998). In both cleavages, Ala is in P1".
Molecular structureThe first tertiary structure to be solved was of the TM1457 protein from Thermotoga maritima. The fold comprises two helices contacting one side of a five-stranded beta sheet. The proposed active site lies in the large cleft between the first alpha helix and the second beta strand (Shin et al., 2005). Because this fold is unlike that of any other peptidase, family C108 is the representative structure of clan CR.
Distribution of family Bacteria details  
Archaea -  
Protozoa -  
Fungi -  
Plants -  
Animals -  
Viruses details  
Biological functionsPeptidases in family C108 are processing endopeptidases, removing N-terminal propeptides.
Statistics for family C108Sequences:833
Identifiers with PDB entries:2
Downloadable files Sequence library (FastA format)
Sequence alignment (FastA format)
Phylogenetic tree (Newick format)
Peptidases and Homologues MEROPS ID Structure
Prp peptidase (Staphylococcus aureus)C108.001Yes
bacteriophage Cp-1 orf13 proteinC108.002-
family C108 non-peptidase homologuesC108.UNW-
family C108 unassigned peptidasesunassignedYes