|Family type peptidase||C59.001 - penicillin V acylase precursor (Lysinibacillus sphaericus), MEROPS Accession MER0020029 (peptidase unit: 4-338)|
|Content of family||Peptidase family C59 contains Cys-dependent Ntn-hydrolases.|
Identifier created: MEROPS 6.4 (24 September 2003)|
Cys-dependent Ntn-hydrolases are only classified as peptidases by virtue of their self-processing activity. The autolysis step produces a mature amidase with Cys1 as the nucleophile. All peptidase activity is lost on conversion to the mature peptidase.
|Active site residues||C4 |
|Active site||The catalytic Cys becomes the new N-terminus following autolysis (see the Alignment).|
|Activities and specificities||The only known peptidase activity is the autolytic cleavage of the penicillin V acylase precursor protein (C59.001).|
|Molecular structure||The mature peptidase is a homotetramer and has a fold that is similar to that of the proteasome (XT01-001). There are two anti-parallel beta sheets sandwiched between two pairs of anti-parallel alpha helices. There are extensions from one of the pairs of alpha helices and also at the C-terminus, which interact with other monomers in the tetramer and provide stability (Suresh et al., 1999).|