Family C60

Family

Summary Holotypes Alignment Tree Genomes Structure Literature

C60A

Summary Holotypes Alignment Tree Genomes Literature

C60B

Summary Holotypes Alignment Tree Genomes Literature

Summary for family C60

Family type peptidaseC60.001 - sortase A (Staphylococcus-type) (Staphylococcus aureus), MEROPS Accession MER0018290 (peptidase unit: 1-206)
Content of familyPeptidase family C60 contains bacterial peptidases with strong transferase activity.
History Identifier created: MEROPS 6.1 (10 January 2003)
Catalytic typeCysteine
Active siteResidues essential for activity include His120 and Cys184 (see the Alignment), but the two residues probably do not form an ion-pair (Connolly et al., 2003). A third active site residue may be Arg197 (Marraffini et al., 2004; Zong et al., 2004).
Activities and specificitiesSortase A (C60.001) cleaves proteins in the LPXTG motif, and then catalyzes the formation of an amide bond between the new C-terminal Thr and a cell wall pentaglycine cross-bridge (Zong et al., 2004). Specificity for the acceptor requires Gly in P1", and prefers Gly in P2" (Huang et al., 2003). Sortase B (C60.002) catalyses a similar reaction, but the consensus cleavage site is NPQTN (Mazmanian et al., 2002). Substrate specificities of many forms of sortase have been described in detail by Comfort & Clubb, 2004. In vitro, an acceptor such as Gly-Gly-Gly can substitute for the natural acceptor, and in the absence of an acceptor hydrolysis take place (Kruger et al., 2004).
InhibitorsSortases are inhibited by compound E-64 (Zong et al., 2004), and also by peptidyl diazomethanes containing the Leu-Pro-Ala-Thr recognition sequence (Scott et al., 2002). Other classes of inhibitors include vinyl sulfones and diarylacrylonitriles (Connolly et al., 2003; Oh et al., 2004).
Molecular structureStructures described for both sortase A and sortase B show a protein fold that had not been seen for any other peptidase, and the sortase A fold is the type structure for clan CL. The core of the protein is an eight-stranded beta-barrel with mainly antiparallel strands that is decorated by five alpha-helices (Zong et al., 2004; Zhang et al., 2004).
ClanCL
Basis of clan assignmentType family of clan CL.
Distribution of family Bacteria details  
Archaea details  
Protozoa -  
Fungi -  
Plants -  
Animals -  
Viruses details  
Biological functionsThe sortases covalently attach a variety of proteins to the exterior of the bacterial cell wall. These proteins include some that contribute to the diseases caused by the pathogenic bacteria involved.
Pharmaceutical and biotech relevanceThere is evidence that inhibition of sortase activity might decrease the virulence of some pathogenic bacteria, so sortases are regarded as targets for antibacterial drugs.
Statistics for family C60Sequences:1956
Identifiers:9
Identifiers with PDB entries:5
Downloadable files Sequence library (FastA format)
Sequence alignment (FastA format)
Phylogenetic tree (Newick format)
Subfamily C60A
Name Peptidase subfamily C60A
Subfamily type peptidase C60.001 - sortase A ({Staphylococcus}-type) (Staphylococcus aureus), MEROPS Accession MER0018290 (peptidase unit: 1-206)
Active site residues H120 C184 
Statistics Sequences: 1380
Identifiers: 7
Identifiers with PDB entries: 4
Other databases CATH 2.40.260.10
HOMSTRAD hs1ijaa
HSSP 1ija
PANTHER PTHR33393
PFAM PF04203
SCOP 63818
Downloadable files Sequence library [FastA format]
Sequence alignment [FastA format]
Phylogenetic tree [Newick format]
Peptidases and Homologues MEROPS ID Structure
sortase A (Staphylococcus-type)C60.001Yes
sortase C (Bacillus-type)C60.004-
sortase DC60.005-
sortase A (Streptococcus-type)C60.006Yes
sortase C1 (Streptococcus sp.)C60.007Yes
sortase yhcS (Escherichia coli)C60.008-
ywpE/BSU36340 (Bacillus subtilis)C60.A01-
subfamily C60A non-peptidase homologuesnon-peptidase homologue-
subfamily C60A unassigned peptidasesunassignedYes
Subfamily C60B
Name Peptidase subfamily C60B
Subfamily type peptidase C60.002 - sortase B (Staphylococcus aureus), MEROPS Accession MER0020433 (peptidase unit: 5-242)
Active site residues H130 C223 
Statistics Sequences: 505
Identifiers: 2
Identifiers with PDB entries: 1
Other databases CATH 2.40.260.10
HOMSTRAD hs1ng5a
PFAM PF04203
PFAM PF07170
SCOP 63818
Downloadable files Sequence library [FastA format]
Sequence alignment [FastA format]
Phylogenetic tree [Newick format]
Peptidases and Homologues MEROPS ID Structure
sortase BC60.002Yes
sortase C2C60.003-
subfamily C60B unassigned peptidasesunassigned-
Peptidases not assigned to subfamily
Peptidases and Homologues MEROPS ID Structure
family C60 non-peptidase homologuesnon-peptidase homologue-
family C60 unassigned peptidasesunassigned-