Family C79


Summary Holotypes Alignment Tree Genomes Literature

Summary for family C79

Family type peptidaseC79.001 - ElaD peptidase (Escherichia-type) (Escherichia coli), MEROPS Accession MER0029945 (peptidase unit: 1-403)
Content of familyPeptidase family C79 contains peptidases with deubiquitinylating activity.
History Identifier created: MEROPS 7.9 (17 September 2007)
ElaD (Escherichia coli) and SseL (Salmonella enterica serovar Typhimurium) were discovered to be deubiquitinylating peptidases in 2007 (see bleow).
Catalytic typeCysteine
Active site residuesH230 C312 
Active siteActive site Cys residues have been identified by mutagenesis (Catic et al., 2007; Rytkonen et al., 2007). The active site His is recognised by similarity to clan CE peptidases (see the Alignment for clan CE).
Activities and specificitiesElaD and the similar SseL from Salmonella enterica serovar Typhimurium hydrolyse ubiquitinyl bonds. Recombinant ElaD hydrolysed ubiquitinyl aminomethylcoumarin and showed other reactions characteristic of a ubiquitinyl hydrolase (Catic et al., 2007). A fusion protein containing SseL hydrolysed mono- and polyubiquitin substrates in vitro with a preference for Lys63-linked ubiquitin chains, but also cleaving at Lys-48 (Rytkonen et al., 2007).
InhibitorsElaD (but not its C312S form) reacted with a potential inhibitor in which a Michael acceptor was added to the C-terminus of ubiquitin (Catic et al., 2007).
Molecular structureNo three-dimensional structure is yet available.
Distribution of family Bacteria details  
Archaea -  
Protozoa -  
Fungi -  
Plants -  
Animals -  
Viruses -  
Biological functions
Statistics for family C79Sequences:90
Identifiers with PDB entries:0
Downloadable files Sequence library (FastA format)
Sequence alignment (FastA format)
Phylogenetic tree (Newick format)
Peptidases and Homologues MEROPS ID Structure
ElaD peptidase (Escherichia-type)C79.001-
non-peptidase homologue-
family C79 unassigned peptidasesunassigned-