|Family type peptidase||C95.001 - lysosomal 66.3 kDa protein (Mus musculus-type) (Mus musculus), MEROPS Accession MER0175067 (peptidase unit: 249-594)|
|Content of family||Family C95 contains self-cleaving proteins.|
Identifier created: MEROPS 9.6 (29 February 2012)|
The mouse 66.3 kDa lysosomal protein (C95.001) is synthesized precursor, which is then processed within the lysosome. Because the structure of the this protein has been solved and is similar to that of Ntn-hydrolases, the initial processing event is presumed to be autolytic (Lakomek et al., 2009).
|Active site||The N-terminal Cys249 of the mature protein is assumed to be the nucleophile responsible for self-processing (Lakomek et al., 2009).|
|Activities and specificities||The only known proteolytic activity is a processing event that is assumed to be the result of autolytic activity. The mouse 66.3 kDa lysosomal protein (C95.001) is cleaved at the Ser248+Cys bond (Deuschl et al., 2006). Processing occurs within the lysosome, where the glycosylated 76 kDa precursor is first processed into an N-terminal 28 kDa chain and a C-terminal 40 kDa chain. The C-terminal chain is further processed into a 15 kDa form with cleavage occuring at Arg514+Ser, presumably by other lysosomal peptidases.|
|Molecular structure||The mouse 66.3 kDa lysosomal protein has been crystallized and shown to have a tertiary structure similar to that of Ntn-hydrolases (Lakomek et al., 2009). Family C95 is thus included in clan PB, subclan PB(C).|