Family C95


Summary Holotypes Alignment Tree Genomes Structure Literature H-seq M-seq

Summary for family C95

Family type peptidaseC95.001 - lysosomal 66.3 kDa protein (Mus musculus-type) (Mus musculus), MEROPS Accession MER0175067 (peptidase unit: 249-594)
Content of familyFamily C95 contains self-cleaving proteins.
History Identifier created: MEROPS 9.6 (29 February 2012)
The mouse 66.3 kDa lysosomal protein (C95.001) is synthesized precursor, which is then processed within the lysosome. Because the structure of the this protein has been solved and is similar to that of Ntn-hydrolases, the initial processing event is presumed to be autolytic (Lakomek et al., 2009).
Catalytic typeCysteine
Active siteThe N-terminal Cys249 of the mature protein is assumed to be the nucleophile responsible for self-processing (Lakomek et al., 2009).
Activities and specificitiesThe only known proteolytic activity is a processing event that is assumed to be the result of autolytic activity. The mouse 66.3 kDa lysosomal protein (C95.001) is cleaved at the Ser248+Cys bond (Deuschl et al., 2006). Processing occurs within the lysosome, where the glycosylated 76 kDa precursor is first processed into an N-terminal 28 kDa chain and a C-terminal 40 kDa chain. The C-terminal chain is further processed into a 15 kDa form with cleavage occuring at Arg514+Ser, presumably by other lysosomal peptidases.
Molecular structureThe mouse 66.3 kDa lysosomal protein has been crystallized and shown to have a tertiary structure similar to that of Ntn-hydrolases (Lakomek et al., 2009). Family C95 is thus included in clan PB, subclan PB(C).
Distribution of family Bacteria details  
Archaea details  
Protozoa details  
Fungi -  
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Biological functions
Statistics for family C95Sequences:795
Identifiers with PDB entries:2
Downloadable files Sequence library (FastA format)
Sequence alignment (FastA format)
Phylogenetic tree (Newick format)
Peptidases and Homologues MEROPS ID Structure
lysosomal 66.3 kDa protein (Mus musculus-type)C95.001Yes
phospholipase B-like protein 1C95.002Yes
non-peptidase homologue-
family C95 unassigned peptidasesunassigned-