Family M30


Summary Holotypes Alignment Tree Genomes Literature

Summary for family M30

NamePeptidase family M30 (hyicolysin family)
Family type peptidaseM30.001 - hyicolysin (Staphylococcus hyicus), MEROPS Accession MER0001182 (peptidase unit: 102-438)
Content of familyFamily M30 contains endopeptidases.
History Identifier created: Methods Enzymol. 248:183-228 (1995)
Only hyicolysin (M30.001), the peptidase from Staphylococcus hyicus, has been characterized (for a review see Götz, 2004).
Catalytic typeMetallo
Active site residuesH242 E243 H246 E269 
Active siteHyicolysin is a metallopeptidase with a single zinc ion, liganded by two histidines and a glutamate. The histidines occur in an HEXXH motif, in which the glutamate is believed to be a catalytic residue.
Activities and specificitiesHyicolysin is active between pH 7.4 and 8.5. The peptidase is able to cleave azocasein and gelatin, but not elastin, FA-Gly-Leu-amide, a synthetic substrate for thermolysin (M04.001) nor FA-Leu-Gly-Pro-Ala, a synthetic substrate for collagenase 1 (M10.001).
InhibitorsHyicolysin is inhibited by the metal chelators EDTA, EGTA and 1,10-phenanthroline. Only zinc and cobalt ions can reactivate the enzyme. Unlike thermolysin, hyicolysin is not inhibited by phosphoramidon.
Molecular structureNo tertiary structure has been determined for any member of the family, but on the basis of the presence of the HEXXH zinc-binding motif, coupled with Glu as the third zinc ligand, family M30 is included in clan MA.
Basis of clan assignmentPredicted active site residues for members of this family and thermolysin, the type example for clan MA, occur in the motif HEXXH
Distribution of family Bacteria details  
Archaea details  
Protozoa -  
Fungi -  
Plants -  
Animals details  
Viruses -  
Biological functionsPhysiological substrates are unknown. Hyicolysin is synthesized as a precursor with an N-terminal signal peptide followed by a propeptide. Maturation is assumed to be by autolysis, and mature hyicolysin is secreted to the extracellular medium.
Pharmaceutical and biotech relevanceStaphylococcus hyicus infects pigs, cattle and chickens. In pigs it is associated with exudative dermatitis, necrosis of the tips of the ears, and tail-biting. Because the hyicolysin gene is present in isolates from infected animals, it is assumed to have a role in pathology and to be of agricultural and veterinary importance.
Statistics for family M30Sequences:143
Identifiers with PDB entries:0
Downloadable files Sequence library (FastA format)
Sequence alignment (FastA format)
Phylogenetic tree (Newick format)
Peptidases and Homologues MEROPS ID Structure
family M30 non-peptidase homologuesnon-peptidase homologue-
family M30 unassigned peptidasesunassigned-