Family M72


Summary Holotypes Alignment Tree Genomes Literature

Summary for family M72

Family type peptidaseM72.001 - peptidyl-Asp metallopeptidase (Pseudomonas aeruginosa), MEROPS Accession MER0022935 (peptidase unit: 27-267)
Content of familyFamily M72 contains metallo-endopeptidases.
History Identifier created: MEROPS 6.3 (23 June 2003)
Although peptidyl-Asp metalloendopeptidase (M72.001) from Pseudomonas fragi has been known for many years, only fragments of amino acid sequence had been determined. The sequencing of two Xanthomonas genomes established the first complete sequence of a member of family M72.
Catalytic typeMetallo
Active site residuesH167 E168 H171 H177 
Active siteOne zinc ion is predicted to be bound by three histidine residues within the motif HEXXHXXGXXH, and the glutamate is assumed to be a catalytic residue.
Activities and specificitiesPeptidyl-Asp metalloendopeptidase has a restricted specificity, cleaving peptide bonds at the amino side of aspartate or cysteic acid (Drapeau, 1980). Cleavage at the amino side of glutamate can occur under certain conditions (Ingrosso et al., 1989; Tetaz et al., 1990). A similar specificity is shown by flavastacin (M12.066) from Flavobacterium meningosepticum.
InhibitorsMetal chelators such as 1,10-phenanthroline, EDTA and EGTA are effective inhibitors.
Molecular structurePeptidyl-Asp metalloendopeptidase is a secreted enzyme and is synthesized with an N-terminal signal peptide. The peptidase unit is the N-terminal half of the mature protein; the C-terminal half contains two carbohydrate binding domains.
Basis of clan assignmentPredicted active site residues for members of this family and thermolysin, the type example for clan MA, occur in the motif HEXXH
Distribution of family Bacteria details  
Archaea -  
Protozoa -  
Fungi -  
Plants -  
Animals -  
Viruses -  
Biological functions
Pharmaceutical and biotech relevanceBecause of the unusual specificity, peptidyl-Asp metalloendopeptidase is commercially available (as 'endoproteinase Asp-N'); it has been used to generate peptides for protein sequencing (Ponstingl et al., 1986) and for the determination of disulfide bridges (Leonard et al., 1990).
Statistics for family M72Sequences:113
Identifiers with PDB entries:0
Downloadable files Sequence library (FastA format)
Sequence alignment (FastA format)
Phylogenetic tree (Newick format)
Peptidases and Homologues MEROPS ID Structure
peptidyl-Asp metallopeptidaseM72.001-
family M72 non-peptidase homologuesnon-peptidase homologue-
family M72 unassigned peptidasesunassigned-