|Family type peptidase||M88.001 - IMPa peptidase (Pseudomonas aeruginosa) (Pseudomonas aeruginosa), MEROPS Accession MER0185642 (peptidase unit: 181-923)|
|Content of family||Family M88 contains endopeptidases.|
Identifier created: MEROPS 9.7 (1 August 2012)|
The human pathogenic bacterium Pseudomonas aeruginosa evades immunological attack by secreting peptidases that degrade components of the host defence system, such as pseudolysin (M04.005) and aeruginolysin (M10.056). IMPa ('immunomodulating protease'; M88.001) is another example of a secreted Pseudomonas peptidase that interferes with the immune system by degrading PSGL-1 (P-selectin glycoprotein ligand 1), which by interacting with selectins mediates the migration of leukocytes over vascular surfaces ('rolling') during the initial stages of inflammation (Bardoel et al., 2012).
|Active site residues||E697 |
|Active site||IMPa is assumed to be a metallopeptidase because of the presence of a well-conserved metal-binding motif, HEXXH, in which the histidines are assumed to be two of the three zinc ligands and the glutamic acid a catalytic residue. The third zinc ligand has not been identified and cannot be predicted from the alignment.|