Family N2

Family

Summary Holotypes Alignment Tree Genomes Structure Literature

Summary for family N2

Family type peptidaseN02.001 - tetravirus coat protein (Nudaurelia capensis omega virus), MEROPS Accession MER0004241 (peptidase unit: 1-644)
Content of familyPeptidase family N2 contains only the endopeptidase of tetraviruses.
History Identifier created: MEROPS 9.3 (7 September 2010)
Catalytic typeAsparagine
Active site residuesE103 N570 
Active siteThe residues that form the catalytic dyad in the active site are Glu103 and Asn570. The cleavage mediated by the enzyme is an autolytic one, and the catalytic Asn is also the P1 residue in the cleavage site.
Activities and specificitiesThe only known activity of family N2 is an autolytic cleavage of Asn570Phe571 releasing a 74-residue fragment from the C-terminus of the coat protein. This cleavage occurs during the late stages of virion assembly. Heterologous expression of the coat protein gene of tetravirus in a baculovirus system results in the spontaneous assembly of virus-like particles (VLPs) that remain uncleaved when purified at neutral pH (Taylor et al., 2005).
Molecular structureThe tertiary structure of the Nuradelia capensis omega virus has been solved (Munshi et al., 1996). The fold of the peptidases of family N2 is similar to that of the known structures from family N1, and the families are therefore grouped together in clan NA.
ClanNA
Basis of clan assignmentProtein fold of the peptidase unit for members of this family that of nodavirus endopeptidase, the type example for clan AB.
Distribution of family Bacteria -  
Archaea -  
Protozoa -  
Fungi -  
Plants -  
Animals -  
Viruses details  
Biological functions
ReviewsReddy et al., 2004
Statistics for family N2Sequences:7
Identifiers:1
Identifiers with PDB entries:2
Downloadable files Sequence library (FastA format)
Sequence alignment (FastA format)
Phylogenetic tree (Newick format)
Peptidases and Homologues MEROPS ID Structure
tetravirus coat proteinN02.001Yes
family N2 non-lyase homologuesnon-peptidase homologue-
family N2 unassigned peptide lyasesunassignedYes