Family U32

Family

Summary Holotypes Alignment Tree Genomes Structure Literature

Summary for family U32

Family type peptidaseU32.001 - collagenase (Porphyromonas-type) (Porphyromonas gingivalis), MEROPS Accession MER0003242 (peptidase unit: 28-330)
Content of familyPeptidase family U32 contains endopeptidases from bacteria.
History Identifier created: Proteolysis in Cell Function, pp13-21, IOS Press, Amsterdam (1997)
Proteolytic activity, usually described as 'collagenase', has been attributed to the product of the prtC gene of Porphyromonas gingivalis.
Catalytic typePeptidase of unknown catalytic type
Active siteNothing is known about the active site, and accordingly the peptidase is assigned to a family of unknown catalytic type.
Activities and specificitiesThe PrtC peptidase (U32.001) was reported to degrade soluble and reconstituted fibrillar type I collagen, heat-denatured type I collagen, and azocoll, but not gelatin or a synthetic collagenase substrate (Kato et al., 1992). Activity of the homologue from Helicobacter pylori (U32.002) has been reported, but not characterised in detail (Kavermann et al., R., 2003).
InhibitorsThe PrtC peptidase was inhibited by EDTA and thiol-blocking agents (Kato et al., 1992).
Molecular structureThe PrtC peptidase ran as a band of 35 kDa in SDS gel electrophoresis, and the native enzyme behaved as a dimer in gel filtration chromatography (Kato et al., 1992). The deduced amino acid sequence shows no relationship to those of peptidases in any other family.
Clanunassigned
Basis of clan assignmentProtein fold and active site residues are not known for any members of this family.
Distribution of family Bacteria details  
Archaea details  
Protozoa -  
Fungi -  
Plants details  
Animals details  
Viruses details  
Biological functions
Pharmaceutical and biotech relevanceHeterogeneity of the PrtC gene of Porphyromonas gingivalis may facilitate the recognition of distinct strains of the organism (Wittstock et al., 2000), and immunoassays for the protein may have diagnostic value in periodontitis (Beikler et al., 2003).
Statistics for family U32Sequences:2729
Identifiers:6
Identifiers with PDB entries:2
Downloadable files Sequence library (FastA format)
Sequence alignment (FastA format)
Phylogenetic tree (Newick format)
Peptidases and Homologues MEROPS ID Structure
collagenase (Porphyromonas-type)U32.001Yes
collagenase (Helicobacter-type)U32.002-
collagenase (Salmonella-type)U32.003-
Mername-AA261 putative peptidase (Clostridium beijerinckii) and similarU32.004-
YhbV protein (Escherichia coli)U32.A01-
yrrN g.p. (Bacillus subtilis)U32.A02-
family U32 unassigned peptidasesunassignedYes