Summary for peptidase A01.020: phytepsin

Summary Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates Inhibitors

 

Names
MEROPS Namephytepsin
Other namesarctiumisin (Arctium minus), At1g11910 (Arabidopsis thaliana), AtPaspA1 (Arabidopsis thaliana), AtPaspA2 (Arabidopsis thaliana), AtPaspA3 (Arabidopsis thaliana), cardosin A (Cynara cardunculus), cardosin B (Cynara cardunculus), cardosin C (Cynara cardunculus), cardosin D (Cynara cardunculus), cenprosin Centauria calcitrapa, cirsin (Cirsium vulgare), CPA63 allergen (Cryptomeria japonica), cynarase (Cynara cardunculus), cyprosin, LeAspP, oryzasin, pra-1 g.p. (Arabidopsis thaliana), salpichroin (Salpichroa origanifolia), soyAP1 (Glycine max), soyAP2 (Glycine max), StAsp (Solanum tuberosum), WAP1 (Triticum aestivum), WAP2 (Triticum aestivum)
Domain architecture
MEROPS Classification
Classification Clan AA >> Subclan (none) >> Family A1 >> Subfamily A >> A01.020
Holotypephytepsin (Hordeum vulgare), Uniprot accession P42210 (peptidase unit: 76-508), MERNUM MER0000949
History Identifier created: Handbook of Proteolytic Enzymes (1998) Academic Press, London.
Activity
Catalytic typeAspartic
PeplistIncluded in the Peplist with identifier PL00017
NC-IUBMBSubclass 3.4 (Peptidases) >> Sub-subclass 3.4.23 (Aspartic endopeptidases) >> Peptidase 3.4.23.40
EnzymologyBRENDA database
Proteolytic eventsCutDB database (16 cleavages)
BiotechnologyContributes to the malting of cereal grains. Forms from Centauria sp. and Cynara sp. have been used to coagulate milk in cheese making (e.g. Silva et al., 2000).
PhysiologyPhytepsin is implicated in the germination of seeds and senescence of plant tissues (Terauchi et al., 2004; de Carvalho et al., 2004).
Cleavage site specificity Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage pattern-/-/-/flScissile bond-/-/-/- (based on 62 cleavages)
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Specificity matrix
 
Amino acid P4 P3 P2 P1 P1' P2' P3' P4'
Gly 2 7 4 1 1 2 0 3
Pro 6 1 0 0 0 3 2 12
Ala 6 3 4 4 3 5 4 1
Val 5 2 6 1 3 3 7 1
Leu 8 14 7 14 4 3 11 6
Ile 0 2 1 1 2 2 6 1
Met 0 0 0 1 5 1 0 0
Phe 2 3 2 21 4 0 1 1
Tyr 3 3 3 2 11 5 2 2
Trp 0 0 2 5 1 0 1 0
Ser 5 4 8 2 3 3 4 1
Thr 0 7 2 0 3 2 4 3
Cys 3 0 1 1 0 2 1 2
Asn 5 2 0 2 0 2 3 3
Gln 0 2 4 1 2 10 3 3
Asp 3 1 4 2 4 4 4 2
Glu 3 5 6 2 2 4 4 1
Lys 1 3 2 0 0 2 3 7
Arg 1 1 4 1 5 7 1 2
His 5 0 1 0 1 1 0 0