Summary for peptidase A01.030: yapsin-1

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates Inhibitors

 

Names
MEROPS Nameyapsin-1
Other namesCgYps1 peptidase (Candida glabrata), YAP3 [obs.] g.p. (Saccharomyces cerevisiae), YPS1 g.p. (Saccharomyces cerevisiae), yeast aspartic protease 3
Domain architecture
MEROPS Classification
Classification Clan AA >> Subclan (none) >> Family A1 >> Subfamily A >> A01.030
Holotypeyapsin-1 (Saccharomyces cerevisiae), Uniprot accession P32329 (peptidase unit: 63-478), MERNUM MER0000948
History Identifier created: Handbook of Proteolytic Enzymes (1998) Academic Press, London.
Activity
Catalytic typeAspartic
PeplistIncluded in the Peplist with identifier PL00020
NC-IUBMBSubclass 3.4 (Peptidases) >> Sub-subclass 3.4.23 (Aspartic endopeptidases) >> Peptidase 3.4.23.41
EnzymologyBRENDA database
Proteolytic eventsCutDB database (4 cleavages)
BiotechnologyCan degrade proteins artificially expressed in Saccharomyces cerevisiae, so genetically-deficient strains may be advantageous (Kerry-Williams et al., 1998).
PhysiologyBasic residue-specific processing of protein precursors in Saccharomyces cerevisiae (Ledgerwood et al., 1996).
Cleavage site specificity Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage patterns/m/i/KRScissile bondnr/l/q/sgl (based on 45 cleavages)
weblogo
Specificity matrix
 
Amino acid P4 P3 P2 P1 P1' P2' P3' P4'
Gly 2 1 1 0 0 5 2 8
Pro 4 2 0 0 0 1 0 2
Ala 2 6 1 1 1 4 0 1
Val 0 7 2 0 0 3 2 2
Leu 0 5 3 1 0 12 3 6
Ile 2 0 14 0 5 1 1 0
Met 0 13 0 3 1 0 2 1
Phe 2 2 0 0 0 1 0 1
Tyr 0 0 2 0 2 3 0 0
Trp 0 1 0 0 0 0 0 0
Ser 14 1 5 0 2 1 2 15
Thr 0 1 0 0 0 0 0 0
Cys 0 0 0 0 0 0 0 0
Asn 4 0 1 0 14 0 5 0
Gln 0 0 1 0 2 0 12 1
Asp 1 1 2 0 1 3 3 2
Glu 3 0 3 0 3 1 7 1
Lys 3 3 5 20 5 2 1 1
Arg 6 2 5 20 8 6 3 0
His 0 0 0 0 0 0 0 0