Summary for peptidase A24.001: type 4 prepilin peptidase 1

Summary Alignment Tree Sequences Sequence features Distribution Literature Substrates Inhibitors

 

Names
MEROPS Nametype 4 prepilin peptidase 1
Other namesCofP g.p. (Escherichia coli), fimP g.p. (Dichelobacter nodosus), gspO g.p. (Escherichia coli), leader peptidase 3, PilD g.p. (Pseudomonas aeruginosa), type IV prepilin peptidase, pppA g.p. (Escherichia coli), PulO g.p. (Klebsiella pneumoniae), tcpJ g.p. (Vibrio cholerae), type four prepilin peptidase, TFPP, VcpD g.p. (Vibrio cholerae), VvpD g.p. (Vibrio vulnificus)
Domain architecture
MEROPS Classification
Classification Clan AD >> Subclan (none) >> Family A24 >> Subfamily A >> A24.001
Holotypetype 4 prepilin peptidase 1 (Pseudomonas aeruginosa), Uniprot accession P22610 (peptidase unit: 50-281), MERNUM MER0000870
History Identifier created: Handbook of Proteolytic Enzymes (1998) Academic Press, London.
Activity
Catalytic typeAspartic
PeplistIncluded in the Peplist with identifier PL00043
NC-IUBMBSubclass 3.4 (Peptidases) >> Sub-subclass 3.4.23 (Aspartic endopeptidases) >> Peptidase 3.4.23.43
EnzymologyBRENDA database
Proteolytic eventsCutDB database (5 cleavages)
PhysiologyThis membrane-bound peptidase cleaves a specialized leader peptide from type 4 prepilin during its secretion from many bacterial species. Once secreted, the processed proteins are required for functions including type 4 pilus formation, toxin and other enzyme secretion, gene transfer, and biofilm formation.
Cleavage site specificity Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage patternar/Q/krs/GScissile bondF/T/L/IL (based on 16 cleavages)
weblogo
Specificity matrix
 
Amino acid P4 P3 P2 P1 P1' P2' P3' P4'
Gly 1 0 0 16 0 0 0 0
Pro 0 0 0 0 0 0 0 0
Ala 4 1 0 0 0 0 0 0
Val 0 0 0 0 0 0 0 1
Leu 3 0 0 0 1 0 15 5
Ile 0 0 0 0 0 0 0 10
Met 0 0 0 0 2 0 1 0
Phe 0 0 0 0 13 0 0 0
Tyr 1 0 0 0 0 0 0 0
Trp 0 0 0 0 0 0 0 0
Ser 0 2 4 0 0 2 0 0
Thr 0 0 0 0 0 14 0 0
Cys 0 0 0 0 0 0 0 0
Asn 0 0 0 0 0 0 0 0
Gln 1 12 0 0 0 0 0 0
Asp 0 0 0 0 0 0 0 0
Glu 0 1 1 0 0 0 0 0
Lys 1 0 6 0 0 0 0 0
Arg 4 0 5 0 0 0 0 0
His 1 0 0 0 0 0 0 0