Summary for peptidase C01.002: chymopapain

Summary Alignment Sequences Sequence features Distribution Structure Literature Substrates Inhibitors

 

Names
MEROPS Namechymopapain
Other nameschymopapain A, chymopapain B
Domain architecture
MEROPS Classification
Classification Clan CA >> Subclan (none) >> Family C1 >> Subfamily A >> C01.002
Holotypechymopapain (Carica papaya), Uniprot accession P14080 (peptidase unit: 135-350), MERNUM MER0000706
History Identifier created: Handbook of Proteolytic Enzymes (1998) Academic Press, London.
Activity
Catalytic typeCysteine
PeplistIncluded in the Peplist with identifier PL00047
NC-IUBMBSubclass 3.4 (Peptidases) >> Sub-subclass 3.4.22 (Cysteine endopeptidases) >> Peptidase 3.4.22.6
EnzymologyBRENDA database
BiotechnologyContact-lens cleaning fluid. Stem cell isolation.
Pharmaceutical relevanceTreatment of herniated intervertebral disk (patent).
Other databases WIKIPEDIAhttp://en.wikipedia.org/wiki/Chymopapain
Cleavage site specificity Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage pattern-/-/vf/rScissile bondg/-/-/- (based on 12 cleavages)
weblogo
Specificity matrix
 
Amino acid P4 P3 P2 P1 P1' P2' P3' P4'
Gly 1 2 0 0 3 0 0 1
Pro 0 2 0 0 0 1 1 0
Ala 2 0 0 0 2 0 0 1
Val 0 0 5 0 0 0 0 0
Leu 0 2 1 0 0 1 1 2
Ile 0 0 0 0 0 0 0 0
Met 0 0 0 0 0 0 0 0
Phe 0 2 3 1 0 1 1 0
Tyr 1 0 0 2 1 0 1 1
Trp 0 0 0 0 0 0 0 0
Ser 0 0 0 0 0 1 0 0
Thr 0 0 0 0 1 1 0 0
Cys 0 0 0 0 0 0 0 0
Asn 0 0 0 1 0 0 0 0
Gln 1 0 0 0 1 0 0 0
Asp 0 0 0 0 0 0 0 0
Glu 0 0 1 1 0 1 0 0
Lys 0 0 0 2 0 0 1 1
Arg 1 0 0 3 0 0 1 0
His 1 1 0 0 0 1 1 0
Specificity from combinatorial peptides
 
Organism comment P4 P3 P2 P1 P1' P2' P3' P4' optimal substrate fluorophore or acceptor-donor pair Reference
Carica papaya wild type - E/I/L M/F/P R/C - - - - xEMR+xxxx ACC-Dabcyl Sun et al., 2007
Carica papaya wild type A F/P/V F/broad K/V/S/F - - - - AFFK+xxxx FAM-TAMRA Diaz-Mochon et al., 2006