Summary for peptidase C01.005: stem bromelain

Summary Alignment Sequences Sequence features Distribution Structure Literature Substrates Inhibitors

 

Names
MEROPS Namestem bromelain
Other namesAnanase, bromelain
Domain architecture
MEROPS Classification
Classification Clan CA >> Subclan (none) >> Family C1 >> Subfamily A >> C01.005
Holotypestem bromelain (Ananas comosus), Uniprot accession P14518 (peptidase unit: 2-211), MERNUM MER0000709
History Identifier created: Handbook of Proteolytic Enzymes (1998) Academic Press, London.
Activity
Catalytic typeCysteine
PeplistIncluded in the Peplist with identifier PL00050
NC-IUBMBSubclass 3.4 (Peptidases) >> Sub-subclass 3.4.22 (Cysteine endopeptidases) >> Peptidase 3.4.22.32
EnzymologyBRENDA database
BiotechnologyLaboratory reagent in blood group determination.
Pharmaceutical relevanceBromelain may be effective for burn debridement (Rowan et al., 1990). It may also be useful to activate inflammatory response or as vaccine adjuvant (Engwerda et al.%, 2001; Engwerda et al.%, 2001). It has been suggested to be useful in the treatment of osteoarthritis (Brien et al., 2004). Bromelain has been shown to improve the decrease in defecation in postoperative rats, and it is suggested that bromelain may benefit patients with postoperative ileus (Wen et al., 2005).
Other databases WIKIPEDIAhttp://en.wikipedia.org/wiki/Bromelain
Cleavage site specificity Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage pattern-/pl/-/-Scissile bond-/-/-/- (based on 26 cleavages)
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Specificity matrix
 
Amino acid P4 P3 P2 P1 P1' P2' P3' P4'
Gly 0 1 3 2 2 0 1 3
Pro 4 6 1 0 0 3 2 2
Ala 1 1 0 2 0 2 0 1
Val 0 1 3 2 2 1 1 1
Leu 2 5 4 3 1 2 3 1
Ile 1 0 0 0 1 0 0 0
Met 0 0 0 0 0 0 0 0
Phe 1 1 3 4 2 0 1 1
Tyr 2 0 1 1 1 1 0 2
Trp 0 0 0 0 0 0 0 0
Ser 2 1 2 5 6 4 3 2
Thr 1 1 1 2 2 1 1 1
Cys 1 0 0 0 0 0 1 0
Asn 0 0 1 1 1 1 2 0
Gln 0 0 0 1 1 1 1 0
Asp 0 0 0 0 1 1 1 1
Glu 2 0 0 0 1 2 2 4
Lys 0 1 0 0 1 2 0 1
Arg 3 2 4 2 0 0 1 2
His 1 1 1 0 2 2 2 1
Specificity from combinatorial peptides
 
Organism comment P4 P3 P2 P1 P1' P2' P3' P4' optimal substrate fluorophore or acceptor-donor pair Reference
Ananas comosus wild type - G/F/V F/L/I R - - - - xGFR+xxxx ACC-Dabcyl Sun et al., 2007
Ananas comosus wild type G/broad P R R/Q/M - - - - GPRR ACC Choe et al., 2006