Summary for peptidase C01.050: histolysain

Summary Alignment Tree Sequences Sequence features Distribution Literature Substrates Inhibitors

 

Names
MEROPS Namehistolysain
Other namesamebapain, amoebapain, ACP2 Entamoeba histolytica, CPNP (Entamoeba histolytica), CPP (Entamoeba histolytica), CPP1 (Entamoeba histolytica), EhCP-A5 (Entamoeba histolytica), EhCP1 g.p. (Entamoeba histolytica), EhCP2 g.p. (Entamoeba histolytica), EhCP3 g.p. (Entamoeba histolytica), EhCP5 g.p. (Entamoeba histolytica), EhCP6 g.p. (Entamoeba histolytica), EhCP8 g.p. (Entamoeba histolytica), EhCP9 g.p. (Entamoeba histolytica), EhCP10 g.p. (Entamoeba histolytica), EhCP17 g.p. (Entamoeba histolytica), EhCP19 g.p. (Entamoeba histolytica), EhCP-A peptidase (Entamoeba histolytica), EhlCP1, histolysin
Domain architecture
MEROPS Classification
Classification Clan CA >> Subclan (none) >> Family C1 >> Subfamily A >> C01.050
Holotypehistolysain (Entamoeba histolytica), Uniprot accession Q01958 (peptidase unit: 98-312), MERNUM MER0000680
History Identifier created: Handbook of Proteolytic Enzymes (1998) Academic Press, London.
Activity
Catalytic typeCysteine
PeplistIncluded in the Peplist with identifier PL00066
NC-IUBMBSubclass 3.4 (Peptidases) >> Sub-subclass 3.4.22 (Cysteine endopeptidases) >> Peptidase 3.4.22.35
EnzymologyBRENDA database
PhysiologyAssists in the invasion of host tissues by the parasitic amoeba. Disrupts colonic mucin, decreasing its protective function (Moncada et al., 2003).
Pharmaceutical relevanceHistolysain is a potential drug target for amoebic dysentery as there is evidence that it is necessary for the survival of trophozoites in vivo (Olivos-Garcia et al., 2004). It can also be used to diagnose infection (Luaces et al., 1992; Luaces et al., 1993).
Pathways KEGGAmoebiasis
Cleavage site specificity Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage pattern-/-/Raf/RScissile bond-/-/-/- (based on 11 cleavages)
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Specificity matrix
 
Amino acid P4 P3 P2 P1 P1' P2' P3' P4'
Gly 1 0 0 2 0 0 1 0
Pro 0 1 0 0 0 1 0 1
Ala 0 0 2 0 0 0 0 0
Val 0 0 0 0 0 0 0 0
Leu 0 0 0 0 1 0 0 0
Ile 0 0 0 0 0 0 0 0
Met 0 0 0 0 0 0 0 0
Phe 0 2 2 0 1 1 0 0
Tyr 0 0 0 0 0 0 1 0
Trp 0 0 0 0 0 0 0 0
Ser 0 0 0 0 0 0 0 0
Thr 0 0 0 0 0 0 0 0
Cys 0 0 0 0 0 0 0 0
Asn 0 0 0 0 0 0 0 0
Gln 0 0 0 0 0 0 0 0
Asp 0 0 0 0 0 0 0 0
Glu 0 1 0 0 0 0 0 0
Lys 0 0 0 0 0 0 0 0
Arg 0 2 7 9 0 0 0 0
His 0 0 0 0 0 0 0 0