Summary for peptidase C04.004: tobacco etch virus NIa peptidase

Summary Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates

 

Names
MEROPS Nametobacco etch virus NIa peptidase
Other namesTEV protease, tobacco etch virus NIa protease
Domain architecture
MEROPS Classification
Classification Clan PA >> Subclan PA(C) >> Family C4 >> Subfamily (none) >> C04.004
Holotypetobacco etch virus NIa peptidase, Uniprot accession P04517 (peptidase unit: 2036-2279), MERNUM MER0000799
History Identifier created: MEROPS 5.3 (4 December 2000)
Activity
Catalytic typeCysteine
PeplistIncluded in the Peplist with identifier PL00088
NC-IUBMBNot yet included in IUBMB recommendations.
Proteolytic eventsCutDB database (6 cleavages)
BiotechnologyThe tobacco etch virus NIa endopeptidase has a sequence specificity (ENLYFQG/S ) that makes it useful in the processing of recombinant fusion proteins (Kapust et al., 2002).
Cleavage site specificity Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage patternV/RY/F/QScissile bondSg/G/T/Rev (based on 14 cleavages)
weblogo
Specificity matrix
 
Amino acid P4 P3 P2 P1 P1' P2' P3' P4'
Gly 0 0 0 0 2 9 0 0
Pro 0 0 0 0 0 0 1 0
Ala 0 0 0 0 1 0 0 0
Val 9 1 0 0 0 0 0 2
Leu 2 0 1 0 0 1 0 0
Ile 2 0 0 0 0 0 0 0
Met 0 0 0 1 1 0 0 0
Phe 0 0 11 0 0 0 0 0
Tyr 0 6 0 0 1 0 0 0
Trp 0 0 0 0 0 0 0 0
Ser 0 0 1 0 8 0 1 0
Thr 0 0 1 0 0 0 9 0
Cys 0 0 0 0 0 0 0 0
Asn 0 0 0 0 0 0 0 1
Gln 0 0 0 13 0 0 0 0
Asp 0 0 0 0 0 1 1 1
Glu 0 0 0 0 0 1 0 2
Lys 1 0 0 0 0 2 2 0
Arg 0 7 0 0 0 0 0 8
His 0 0 0 0 1 0 0 0