Summary for peptidase C14.004: caspase-7

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Human EST Mouse EST Substrates Inhibitors Pharma

 

Names
MEROPS Namecaspase-7
Other namesICE-LAP3, Mch3, CMH-1
Domain architecture
MEROPS Classification
Classification Clan CD >> Subclan (none) >> Family C14 >> Subfamily A >> C14.004
Holotypecaspase-7 (Homo sapiens), Uniprot accession P55210 (peptidase unit: 62-336), MERNUM MER0002705
History Identifier created: MEROPS 3.02 (25 June 1998)
Activity
Catalytic typeCysteine
PeplistIncluded in the Peplist with identifier PL00101
NC-IUBMBSubclass 3.4 (Peptidases) >> Sub-subclass 3.4.22 (Cysteine endopeptidases) >> Peptidase 3.4.22.60
EnzymologyBRENDA database
Proteolytic eventsCutDB database (52 cleavages)
Activity statushuman: active (Nicholson & Thornberry, 2004)
mouse: active (by similarity) (Lee et al., 2001)
PhysiologyOne of the "executioner" caspases in mammalian cell apoptosis.
Pharmaceutical relevanceA non-peptidic inhibitor of caspase-3 and caspase-7 was effective in reducing cardiac ischemic injury in a rabbit model (Chapman et al., 2002).
Pathways KEGGAlzheimer's disease
KEGGApoptosis
KEGGLegionellosis
KEGGNon-alcoholic fatty liver disease (NAFLD)
KEGGPertussis
KEGGTNF signaling pathway
Other databases WIKIPEDIAhttp://en.wikipedia.org/wiki/Caspase_7
Cleavage site specificity Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage patterndes/e/vp/DScissile bondGas/-/-/- (based on 179 cleavages)
weblogo
Specificity matrix
 
Amino acid P4 P3 P2 P1 P1' P2' P3' P4'
Gly 4 4 3 0 88 16 10 15
Pro 3 0 31 0 1 11 11 8
Ala 9 15 6 0 25 21 18 16
Val 7 13 43 0 1 8 34 12
Leu 3 21 21 0 9 16 16 19
Ile 2 3 14 0 2 5 3 23
Met 4 2 3 0 3 1 6 1
Phe 1 1 3 0 6 4 1 4
Tyr 1 2 5 0 3 2 2 3
Trp 1 0 2 0 0 1 1 0
Ser 19 16 3 0 22 17 14 13
Thr 3 6 23 0 3 8 6 13
Cys 0 0 2 1 3 0 2 4
Asn 0 2 0 0 5 8 6 1
Gln 1 13 3 0 0 23 8 8
Asp 73 11 1 178 1 2 12 6
Glu 28 57 5 0 1 4 11 15
Lys 1 3 2 0 0 25 13 7
Arg 0 0 5 0 2 2 0 5
His 2 2 1 0 2 3 3 4
Specificity from combinatorial peptides
 
Organism comment P4 P3 P2 P1 P1' P2' P3' P4' optimal substrate fluorophore or acceptor-donor pair Reference
Homo sapiens recombinant D - - D G - - - DxxD+Gxxx Abz-Tyr(NO2) Stennicke et al., 2000
Homo sapiens recombinant D E/V V/I D - - - - DEVD AMC Thornberry et al., 1997
Human genetics
Gene symbol Locus Megabases Ensembl Entrez gene Gene Cards OMIM
CASP7 10q25.1-q25.2 ENSG00000165806 840 CASP7 601761
Mouse genetics
Gene symbol Position Megabases Ensembl Entrez gene MGI
Casp7 19:D2 ENSMUSG00000025076 12369 MGI:109383