Summary for peptidase C30.005: SARS coronavirus picornain 3C-like peptidase

Summary Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates Inhibitors Pharma

 

Names
MEROPS NameSARS coronavirus picornain 3C-like peptidase
Other namesM(pro) (SARS coronavirus), SARS virus 3C-like peptidase, SARS virus chymotrypsin-like cysteine peptidase, SARS-CoV main proteinase, severe acute respiratory syndrome coronavirus main protease
Domain architecture
MEROPS Classification
Classification Clan PA >> Subclan PA(C) >> Family C30 >> Subfamily (none) >> C30.005
HolotypeSARS coronavirus picornain 3C-like peptidase, Uniprot accession P0C6U8 (peptidase unit: 3241-3546), MERNUM MER0022793
History Identifier created: MEROPS 6.5 (22 December 2003)
Activity
Catalytic typeCysteine
PeplistIncluded in the Peplist with identifier PL00434
NC-IUBMBSubclass 3.4 (Peptidases) >> Sub-subclass 3.4.22 (Cysteine endopeptidases) >> Peptidase 3.4.22.69
EnzymologyBRENDA database
StructureActive in vitro only as the homodimer (Fan et al., 2003).
Cleavage site specificity Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage patternLav/-/GL/GQScissile bondasg/-/f/a (based on 26 cleavages)
weblogo
Specificity matrix
 
Amino acid P4 P3 P2 P1 P1' P2' P3' P4'
Gly 0 1 13 13 2 3 1 0
Pro 2 0 0 0 0 0 0 0
Ala 5 1 0 0 5 1 1 4
Val 4 3 1 0 0 1 0 1
Leu 13 2 9 0 0 1 0 1
Ile 0 0 0 0 0 1 0 0
Met 0 0 1 0 0 0 1 0
Phe 0 1 2 0 0 0 4 0
Tyr 0 1 0 0 0 0 0 0
Trp 0 1 0 0 0 0 0 0
Ser 0 1 0 0 5 1 0 2
Thr 2 5 0 0 0 0 0 0
Cys 0 0 0 0 0 0 0 0
Asn 0 1 0 0 1 1 2 1
Gln 0 1 0 13 0 0 1 0
Asp 0 0 0 0 0 0 1 0
Glu 0 0 0 0 0 1 2 0
Lys 0 3 0 0 0 3 0 2
Arg 0 3 0 0 0 0 0 2
His 0 1 0 0 0 0 0 0
Specificity from combinatorial peptides
 
Organism comment P4 P3 P2 P1 P1' P2' P3' P4' optimal substrate fluorophore or acceptor-donor pair Reference
West Nile virus recombinant L K/R/L/broad G G - - - - LKGG ACC Drag et al., 2008
SARS coronavirus recombinant V/T/A/S V/W/K L H/Q - - - - VVLH ACC Goetz et al., 2007