Summary for peptidase C54.001: ATG4 peptidase

Summary Alignment Tree Sequences Sequence features Distribution Literature Substrates


MEROPS NameATG4 peptidase
Other namesApg4 protein (Saccharomyces cerevisiae), ATG4 peptidase (Saccharomyces cerevisiae), Aut2 peptidase (Saccharomyces cerevisiae), YNW3 protein (Saccharomyces cerevisiae)
Domain architecture
MEROPS Classification
Classification Clan CA >> Subclan (none) >> Family C54 >> Subfamily (none) >> C54.001
HolotypeATG4 peptidase (Saccharomyces cerevisiae), Uniprot accession P53867 (peptidase unit: 74-380), MERNUM MER0013559
History Identifier created: MEROPS 5.3 (4 December 2000)
Catalytic typeCysteine
NC-IUBMBNot yet included in IUBMB recommendations.
Proteolytic eventsCutDB database (2 cleavages)
PhysiologyIn autophagy, material is transported from the cytoplasm to the vacuole via double-membrane vesicles. The Saccharomyces cerevisiae g.p. Apg8/Aut7 plays an important part in the formation of such vesicles, tending to bind to membranes in spite of its hydrophilic nature. The C-terminal Arg residue of newly synthesized Apg8 is removed by Apg4/Aut2, and a Gly residue becomes the C-terminal residue of the protein that is now designated Apg8FG. Subsequently, Apg8FG forms a conjugate with an unidentified molecule 'X' and thereby binds tightly to membranes (Kirisako et al., 2000).
Pathways KEGGRegulation of autophagy