Summary for peptidase M01.001: aminopeptidase N

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Human EST Mouse EST Substrates Inhibitors Pharma

 

Names
MEROPS Nameaminopeptidase N
Other namesalanine aminopeptidase, amino-oligopeptidase, ANPEP g.p. (Homo sapiens), AOP, aminopeptidase M, CD13, membrane Cys-Gly dipeptidase, membrane aminopeptidase I, membrane alanyl aminopeptidase (eukaryote), microsomal aminopeptidase, neutral aminopeptidase, particle-bound aminopeptidase, peptidase E, pseudo leucine aminopeptidase
Domain architecture
MEROPS Classification
Classification Clan MA >> Subclan MA(E) >> Family M1 >> Subfamily (none) >> M01.001
Holotypeaminopeptidase N (Homo sapiens), Uniprot accession P15144 (peptidase unit: 301-517), MERNUM MER0000997
History Identifier created: Handbook of Proteolytic Enzymes (1998) Academic Press, London.
Activity
Catalytic typeMetallo
PeplistIncluded in the Peplist with identifier PL00123
NC-IUBMBSubclass 3.4 (Peptidases) >> Sub-subclass 3.4.11 (Aminopeptidases) >> Peptidase 3.4.11.2
EnzymologyBRENDA database
Activity statushuman: active (Turner, 2004)
mouse: active (Matthews et al., 2001)
PhysiologyEctoenzyme inactivating enkephalins (Le Guen et al., 2003) and other bioactive peptides. A receptor for coronavirus infection (Wentworth et al., 2001).
KnockoutDogs showing specific deficiency of brush border aminopeptidase N in the small intestines were clinically healthy, and the deficiency was attributed to diminished expression of an otherwise normal enzyme protein (Pemberton et al., 1997).
Pharmaceutical relevanceDegrades bioactive peptides including enkephalins, and hence a drug target for analgesia (Bernkop-Schnurch & Marschutz, 1997). Serum level greatly increased in cholestasis (Janas et al., 2002). In inflammation, induces chemotactic migration of T lymphocytes by its enzymatic activity (Shimizu et al., 2002).
Pathways KEGGGlutathione metabolism
KEGGHematopoietic cell lineage
KEGGRenin-angiotensin system
Other databases WIKIPEDIAhttp://en.wikipedia.org/wiki/Alanine_aminopeptidase
Cleavage site specificity Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage pattern-/-/-/-Scissile bondwgf/y/fi/- (based on 42 cleavages)
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Specificity matrix
 
Amino acid P4 P3 P2 P1 P1' P2' P3' P4'
Gly 0 0 0 3 5 2 0 1
Pro 0 0 0 2 0 0 0 0
Ala 0 0 0 7 0 0 0 0
Val 0 0 0 1 3 1 0 0
Leu 0 0 0 5 3 0 0 2
Ile 0 0 0 0 0 0 3 1
Met 0 0 0 1 0 0 0 1
Phe 0 0 0 3 4 2 3 0
Tyr 0 0 0 3 2 3 1 0
Trp 0 0 0 2 7 0 0 0
Ser 0 0 0 1 0 0 0 0
Thr 0 0 0 0 0 0 0 0
Cys 0 0 0 2 0 1 0 0
Asn 0 0 0 2 0 0 0 1
Gln 0 0 0 2 1 0 0 0
Asp 0 0 0 0 0 0 0 0
Glu 0 0 0 1 0 0 0 0
Lys 0 0 0 1 0 0 1 0
Arg 0 0 0 5 1 0 0 0
His 0 0 0 1 0 0 0 3
Human genetics
Gene symbol Locus Megabases Ensembl Entrez gene Gene Cards OMIM
ANPEP 15q25-q26 ENSG00000166825 290 ANPEP 151530
Mouse genetics
Gene symbol Position Megabases Ensembl Entrez gene MGI
Anpep 7:D1 ENSMUSG00000039062 16790 MGI:96749