Summary for peptidase M02.001: angiotensin-converting enzyme peptidase unit 1

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Human EST Mouse EST Substrates Inhibitors Pharma

 

Names
MEROPS Nameangiotensin-converting enzyme peptidase unit 1
Other namespeptidyl-dipeptidase A, N domain, peptidyl-dipeptidase A peptidase unit 1
Domain architecture
MEROPS Classification
Parent M02.001 is one peptidase unit of the compound peptidase XM02-001. Other units of XM02-001 are M02.004
Classification Clan MA >> Subclan MA(E) >> Family M2 >> Subfamily (none) >> M02.001
Holotypeangiotensin-converting enzyme peptidase unit 1 (Homo sapiens), Uniprot accession P12821 (peptidase unit: 30-583), MERNUM MER0004967
History Identifier created: Handbook of Proteolytic Enzymes (1998) Academic Press, London.
Activity
Catalytic typeMetallo
PeplistIncluded in the Peplist with identifier PL00132
NC-IUBMBSubclass 3.4 (Peptidases) >> Sub-subclass 3.4.15 (Peptidyl-dipeptidases) >> Peptidase 3.4.15.1
EnzymologyBRENDA database
Activity statushuman: active (Araujo et al., 2000)
mouse: active (Vazeux et al., 2001)
PhysiologyThis is peptidase unit 1 of two peptidase units in the somatic form of angiotensin-converting enzyme. Angiotensin-converting enzyme is an ectoenzyme active in conversion of angiotensin I to hypertensive angiotensin II, and degradation of bradykinin and other bioactive peptides. An insertion/deletion polymorphism is associated with left ventricular hypertrophy.
KnockoutEsther et al. (1997) used targeted homologous recombination to create mice expressing a form of the enzyme that contains only peptidase unit 1. The modified enzyme was catalytically active but entirely secreted from cells. Mice had significant plasma activity but no tissue-bound enzyme. They had low blood pressure, renal vascular thickening, and a urine concentrating defect, and were very similar to completely deficient mice (Esther et al., 1996) except that the renal pathology was less severe.
Pharmaceutical relevanceThe somatic form of angiotensin-converting enzyme containing this peptidase unit together with peptidase unit 2 (M02.004) is a drug target for the control of hypertension.
Human genetics
Gene symbol Locus Megabases Ensembl Entrez gene Gene Cards OMIM
ACE 17q23 ENSG00000159640 1636 ACE 106180
Mouse genetics
Gene symbol Position Megabases Ensembl Entrez gene MGI
Ace 11:E1 ENSMUSG00000020681 11421 MGI:87874