Summary for peptidase M04.001: thermolysin

Summary Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates Inhibitors Pharma

 

Names
MEROPS Namethermolysin
Other namesgentlyase, neutral proteinase (Bacillus thermoproteolyticus), megateriopeptidase (Bacillus megaterium), NprS g.p. (Geobacillus stearothermophilus), nprT g.p. (Bacillus thermoproteolyticus), Mername-AA133 peptidase, nprM g.p. (Bacillus megaterium)
Domain architecture
MEROPS Classification
Classification Clan MA >> Subclan MA(E) >> Family M4 >> Subfamily (none) >> M04.001
Holotypethermolysin (Bacillus thermoproteolyticus), Uniprot accession P00800 (peptidase unit: 233-548), MERNUM MER0001026
History Identifier created: Handbook of Proteolytic Enzymes (1998) Academic Press, London.
Activity
Catalytic typeMetallo
PeplistIncluded in the Peplist with identifier PL00143
NC-IUBMBSubclass 3.4 (Peptidases) >> Sub-subclass 3.4.24 (Metalloendopeptidases) >> Peptidase 3.4.24.27
EnzymologyBRENDA database
Proteolytic eventsCutDB database (16 cleavages)
PhysiologyBacterial nutrition.
Cleavage site specificity Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage pattern-/-/-/-Scissile bondlvif/-/-/- (based on 280 cleavages)
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Specificity matrix
 
Amino acid P4 P3 P2 P1 P1' P2' P3' P4'
Gly 25 11 28 36 5 24 23 24
Pro 17 13 8 12 2 3 13 19
Ala 26 25 28 26 21 46 22 18
Val 19 29 26 8 52 27 13 23
Leu 11 22 14 19 97 20 13 24
Ile 12 8 7 5 36 8 10 16
Met 3 3 2 2 4 1 7 3
Phe 6 11 8 11 34 6 15 5
Tyr 9 5 9 6 7 11 8 7
Trp 2 4 1 6 2 3 5 5
Ser 16 15 17 19 6 16 15 16
Thr 12 24 20 30 7 24 17 15
Cys 7 5 4 5 0 2 7 4
Asn 12 9 9 19 1 12 8 10
Gln 8 6 10 11 0 5 11 4
Asp 23 14 7 19 3 15 11 14
Glu 10 19 18 11 1 10 11 10
Lys 13 13 21 21 1 21 19 13
Arg 8 7 15 9 0 11 10 7
His 4 7 7 5 1 4 10 7
Specificity from combinatorial peptides
 
Organism comment P4 P3 P2 P1 P1' P2' P3' P4' optimal substrate fluorophore or acceptor-donor pair Reference
Bacillus thermoproteolyticus recombinant - F/V/A K/Y F/broad L G/R K - xFKF+LGKx D-A2pr (Nma) - Dnp Oda et al., 2005
Bacillus thermoproteolyticus recombinant - - - K - - - - xxxK+xxxx ACC-Dabcyl Sun et al., 2007