Summary for peptidase M04.003: vibriolysin

Summary Alignment Tree Sequences Sequence features Distribution Literature Substrates Inhibitors

 

Names
MEROPS Namevibriolysin
Other namesAeromonas proteolytica neutral proteinase, aeromonolysin, ahyB g.p. (Aeromonas hydrophila), EmpA peptidase (Vibrio anguillarum), hap g.p. (Vibrio cholerae), haemagglutinin proteinase, non-membrane-damaging cytotoxin, MCP-02 (Pseudoalteromonas sp.), vEP peptidase Vibrio vulnificus, vEP-45 (Vibrio vulnificus), VtpA (Vibrio tubiashii), VvP protease Vibrio vulnificus, VvpE g.p. Vibrio vulnificus
Domain architecture
MEROPS Classification
Classification Clan MA >> Subclan MA(E) >> Family M4 >> Subfamily (none) >> M04.003
Holotypevibriolysin (Vibrio proteolyticus), Uniprot accession Q00971 (peptidase unit: 202-499), MERNUM MER0001043
History Identifier created: Handbook of Proteolytic Enzymes (1998) Academic Press, London.
Activity
Catalytic typeMetallo
PeplistIncluded in the Peplist with identifier PL00144
NC-IUBMBSubclass 3.4 (Peptidases) >> Sub-subclass 3.4.24 (Metalloendopeptidases) >> Peptidase 3.4.24.25
EnzymologyBRENDA database
Proteolytic eventsCutDB database (4 cleavages)
PhysiologyPlays a role in attachment of the bacterium to intestinal epithelium. May activate Vibrio toxins.
Pharmaceutical relevanceDamage to the connective tissues caused by V. vulnificus is at least partly attributable to this peptidase (Chuan et al., 1997). Has hemagglutinating activity that seems to be mediated by both the N-terminal peptidase unit and the C-terminal domain of the protein (Miyoshi et al., 1999).
Cleavage site specificity Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage pattern-/-/-/-Scissile bondfla/-/sg/- (based on 17 cleavages)
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Specificity matrix
 
Amino acid P4 P3 P2 P1 P1' P2' P3' P4'
Gly 1 1 1 5 0 1 3 3
Pro 2 1 0 1 0 0 1 1
Ala 3 1 0 1 3 1 2 1
Val 1 2 1 1 1 3 0 0
Leu 0 1 0 0 4 0 0 1
Ile 0 0 0 0 2 0 0 0
Met 0 1 0 0 1 1 0 0
Phe 0 1 1 0 5 0 0 0
Tyr 0 0 0 1 0 0 0 0
Trp 0 0 0 0 0 0 0 0
Ser 1 1 2 2 1 2 4 0
Thr 1 0 2 2 0 2 0 1
Cys 0 0 0 0 0 0 0 1
Asn 0 0 1 0 0 1 1 1
Gln 0 1 3 0 0 1 0 1
Asp 0 0 0 1 0 0 0 2
Glu 1 0 0 0 0 1 2 1
Lys 2 1 0 0 0 0 0 0
Arg 1 1 2 2 0 0 0 0
His 0 1 0 1 0 0 0 0