Summary for peptidase M08.001: leishmanolysin

Summary Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates Inhibitors

 

Names
MEROPS Nameleishmanolysin
Other namesGp63 g.p. (Leishmania sp.), major surface protein (Msp), promastigote surface endopeptidase, promastigote surface protease, PSP, TbMSP-A (Trypanosoma brucei), TbMSP-B (Trypanosoma brucei), TbMSP-C (Trypanosoma brucei)
Domain architecture
MEROPS Classification
Classification Clan MA >> Subclan MA(M) >> Family M8 >> Subfamily (none) >> M08.001
Holotypeleishmanolysin (Leishmania major), Uniprot accession P08148 (peptidase unit: 101-602), MERNUM MER0001165
History Identifier created: Handbook of Proteolytic Enzymes (1998) Academic Press, London.
Activity
Catalytic typeMetallo
PeplistIncluded in the Peplist with identifier PL00151
NC-IUBMBSubclass 3.4 (Peptidases) >> Sub-subclass 3.4.24 (Metalloendopeptidases) >> Peptidase 3.4.24.36
EnzymologyBRENDA database
Proteolytic eventsCutDB database (4 cleavages)
PhysiologyCleaves CD4 on surface of T lymphocytes. Protects promastigotes from lysis by complement. Facilitates migration of the parasite through extracellular matrix (McGwire et al., 2003).
Pharmaceutical relevanceBeing the most abundant protein of the surface membrane of the promastigote form of Leishmania sp., leishmanolysin is a target for vaccine production. The activity of the enzyme may well also contribute to infection of the host, so inhibitors may have therapeutic value (McGwire et al., 2003; Yao et al., 2003).
Pathways KEGGAfrican trypanosomiasis
Cleavage site specificity Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage patternl/-/al/yScissile bondls/kr/k/- (based on 20 cleavages)
weblogo
Specificity matrix
 
Amino acid P4 P3 P2 P1 P1' P2' P3' P4'
Gly 0 0 0 2 0 2 0 2
Pro 0 1 0 0 0 0 0 2
Ala 2 1 5 0 0 0 1 4
Val 0 1 0 3 2 1 0 3
Leu 6 2 5 0 7 0 1 1
Ile 1 4 0 0 1 0 0 1
Met 0 0 0 0 0 1 0 0
Phe 1 2 1 0 2 0 0 1
Tyr 0 2 2 7 0 0 1 0
Trp 0 0 0 1 0 1 0 1
Ser 2 1 3 2 6 1 0 0
Thr 1 0 0 0 1 0 0 2
Cys 0 0 0 0 0 0 0 0
Asn 0 0 0 0 1 0 2 0
Gln 0 0 1 0 0 0 0 0
Asp 0 0 1 1 0 0 2 0
Glu 1 0 0 0 0 1 2 1
Lys 2 1 0 1 0 9 9 1
Arg 1 3 1 3 0 3 1 1
His 1 0 0 0 0 1 0 0