Summary for peptidase M10.003: matrix metallopeptidase-2

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Human EST Mouse EST Substrates Inhibitors Pharma

 

Names
MEROPS Namematrix metallopeptidase-2
Other names3/4 collagenase, 72 kDa gelatinase, matrix metalloproteinase 2, gelatinase A, MMP-2, MMP2, matrix metalloproteinase 5, tissue gelatinase, type IV collagenase
Domain architecture
MEROPS Classification
Classification Clan MA >> Subclan MA(M) >> Family M10 >> Subfamily A >> M10.003
Holotypematrix metallopeptidase-2 (Homo sapiens), Uniprot accession P08253 (peptidase unit: 108-461), MERNUM MER0001080
History Identifier created: Handbook of Proteolytic Enzymes (1998) Academic Press, London.
Activity
Catalytic typeMetallo
PeplistIncluded in the Peplist with identifier PL00156
NC-IUBMBSubclass 3.4 (Peptidases) >> Sub-subclass 3.4.24 (Metalloendopeptidases) >> Peptidase 3.4.24.24
EnzymologyBRENDA database
Proteolytic eventsCutDB database (116 cleavages)
Activity statushuman: active (Murphy, 2004)
mouse: active (by similarity) (Reponen et al., 1992)
PhysiologyRole in turnover of extracellular matrix proteins.
KnockoutMice lacking gelatinase A and also membrane-type matrix metalloproteinase 1 die immediately after birth (Oh et al., 2004). In humans, mutations of gelatinase A can lead to Al-Aqeel Sewairi syndrome, an autosomal recessive disorder with multicentric osteolysis, nodulosis and arthropathy (Al Aqeel, 2005).
Pharmaceutical relevanceDrug target for prevention of pathological tissue damage. Many reports implicate the enzyme in malignant invasion. A mutation causes a multicentric osteolysis and arthritis syndrome (Martignetti et al., 2001).
Pathways KEGGBladder cancer
KEGGEstrogen signaling pathway
KEGGGnRH signaling pathway
KEGGLeukocyte transendothelial migration
KEGGPathways in cancer
KEGGProteoglycans in cancer
Other databases WIKIPEDIAhttp://en.wikipedia.org/wiki/MMP2
Cleavage site specificity Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage pattern-/p/-/-Scissile bondli/-/-/- (based on 3417 cleavages)
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Specificity matrix
 
Amino acid P4 P3 P2 P1 P1' P2' P3' P4'
Gly 252 121 430 482 60 125 590 290
Pro 196 769 70 210 8 15 29 236
Ala 253 368 642 506 110 235 538 332
Val 199 447 129 45 280 444 274 272
Leu 259 364 178 81 1389 390 112 297
Ile 159 288 70 33 557 351 81 168
Met 82 100 44 67 150 97 47 59
Phe 128 81 48 58 177 143 100 126
Tyr 97 51 48 54 131 91 61 53
Trp 11 16 9 39 44 42 25 23
Ser 178 116 483 333 79 229 486 235
Thr 145 93 86 112 61 252 221 162
Cys 37 42 51 39 70 24 11 40
Asn 88 67 130 376 35 120 167 139
Gln 166 79 226 152 129 220 128 149
Asp 148 12 87 206 9 25 161 278
Glu 169 42 161 266 33 123 147 241
Lys 169 54 180 133 66 309 171 190
Arg 138 26 192 105 13 99 26 38
His 92 80 52 120 14 81 42 76
Human genetics
Gene symbol Locus Megabases Ensembl Entrez gene Gene Cards OMIM
MMP2 16q13 ENSG00000087245 4313 MMP2 120360
Mouse genetics
Gene symbol Position Megabases Ensembl Entrez gene MGI
Mmp2 8:C5 ENSMUSG00000031740 17390 MGI:97009