Summary for peptidase M10.005: matrix metallopeptidase-3

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Human EST Mouse EST Substrates Inhibitors Pharma

 

Names
MEROPS Namematrix metallopeptidase-3
Other namescollagenase activating protein, matrix metalloproteinase 3, MMP-3, matrix metalloproteinase 6, procollagenase activator, proteoglycanase, stromelysin 1, transin
Domain architecture
MEROPS Classification
Classification Clan MA >> Subclan MA(M) >> Family M10 >> Subfamily A >> M10.005
Holotypematrix metallopeptidase-3 (Homo sapiens), Uniprot accession P08254 (peptidase unit: 98-279), MERNUM MER0001068
History Identifier created: Handbook of Proteolytic Enzymes (1998) Academic Press, London.
Activity
Catalytic typeMetallo
PeplistIncluded in the Peplist with identifier PL00158
NC-IUBMBSubclass 3.4 (Peptidases) >> Sub-subclass 3.4.24 (Metalloendopeptidases) >> Peptidase 3.4.24.17
EnzymologyBRENDA database
Proteolytic eventsCutDB database (152 cleavages)
Activity statushuman: active (Nagase, 2004)
mouse: active (Matrisian et al., 1986)
PhysiologyRole in turnover of extracellular matrix proteins. Activation of proforms of other matrix metalloendopeptidases.
KnockoutTargeted disruption of the stromelysin-1 gene in mice did not prevent or reduce cartilage destruction in collagen-induced arthritis (Mudgett et al., 1998). The mice show a delay in excisional wound healing due to a failure in wound contraction resulting from impaired contraction of fibroblasts (Bullard et al., 1999a; Bullard et al., 1999b). Lung damage in response to immunoglobulin G immune complexes was alleviated in the deficient mice, indicating that stromelysin-1 is one of the damaging factors in this condition (Warner et al., 2001).
Pharmaceutical relevanceDrug target for prevention of pathological tissue damage.
Pathways KEGGRheumatoid arthritis
KEGGTNF signaling pathway
KEGGTranscriptional misregulation in cancer
Other databases WIKIPEDIAhttp://en.wikipedia.org/wiki/MMP3
Cleavage site specificity Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage pattern-/pa/a/-Scissile bondl/-/-/- (based on 180 cleavages)
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Specificity matrix
 
Amino acid P4 P3 P2 P1 P1' P2' P3' P4'
Gly 30 5 24 36 2 3 36 15
Pro 8 60 13 15 0 2 2 17
Ala 17 34 45 25 13 7 27 13
Val 12 18 10 4 17 18 9 14
Leu 12 18 12 5 42 12 16 12
Ile 6 4 5 0 19 7 4 4
Met 1 6 1 2 11 5 2 2
Phe 7 3 4 2 14 6 4 8
Tyr 2 1 3 2 12 2 1 1
Trp 2 1 1 0 1 4 1 1
Ser 8 7 16 16 10 13 11 12
Thr 9 6 4 5 4 14 10 11
Cys 5 0 2 0 1 0 3 3
Asn 5 3 4 8 6 8 4 7
Gln 11 3 10 8 8 10 5 8
Asp 13 1 3 7 1 3 7 11
Glu 7 2 11 27 6 8 14 15
Lys 5 2 2 8 7 18 8 13
Arg 8 4 8 6 1 25 13 9
His 7 2 1 4 1 11 2 1
Human genetics
Gene symbol Locus Megabases Ensembl Entrez gene Gene Cards OMIM
MMP3 11q23 ENSG00000175007 4314 MMP3 185250
Mouse genetics
Gene symbol Position Megabases Ensembl Entrez gene MGI
Mmp3 9:A1 ENSMUSG00000043613 17392 MGI:97010