Summary for peptidase M12.001: astacin

Summary Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates Inhibitors

 

Names
MEROPS Nameastacin
Other namesAstacus proteinase, crayfish small-molecule proteinase
Domain architecture
MEROPS Classification
Classification Clan MA >> Subclan MA(M) >> Family M12 >> Subfamily A >> M12.001
Holotypeastacin (Astacus astacus), Uniprot accession P07584 (peptidase unit: 56-243), MERNUM MER0001104
History Identifier created: Handbook of Proteolytic Enzymes (1998) Academic Press, London.
Activity
Catalytic typeMetallo
PeplistIncluded in the Peplist with identifier PL00173
NC-IUBMBSubclass 3.4 (Peptidases) >> Sub-subclass 3.4.24 (Metalloendopeptidases) >> Peptidase 3.4.24.21
EnzymologyBRENDA database
PhysiologyDigestion of food in Astacus.
Cleavage site specificity Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage pattern-/-/-/-Scissile bondasgt/-/-/- (based on 209 cleavages)
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Specificity matrix
 
Amino acid P4 P3 P2 P1 P1' P2' P3' P4'
Gly 22 12 14 23 50 22 20 21
Pro 14 22 13 6 2 42 18 13
Ala 20 20 20 23 51 18 14 9
Val 9 28 13 1 9 15 25 18
Leu 14 16 29 11 4 12 18 12
Ile 9 17 11 1 1 9 20 19
Met 4 2 8 5 0 2 3 3
Phe 5 2 9 13 1 4 9 12
Tyr 2 2 5 10 0 1 2 5
Trp 3 3 1 5 0 1 1 3
Ser 11 13 13 23 51 12 10 7
Thr 11 9 13 2 22 19 14 12
Cys 5 1 6 0 3 2 1 3
Asn 5 4 14 19 7 6 7 9
Gln 9 11 17 11 3 14 6 6
Asp 12 4 5 17 0 18 14 11
Glu 5 15 8 20 1 8 10 12
Lys 28 19 5 6 0 0 6 22
Arg 12 6 3 6 2 0 5 4
His 6 2 2 7 1 3 4 7