Summary for peptidase M12.221: ADAMTS4 peptidase

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Human EST Mouse EST Substrates Inhibitors Pharma

 

Names
MEROPS NameADAMTS4 peptidase
Other namesADAMTS4 g.p. (Homo sapiens), aggrecanase-1, ADAMTS-2 (Tang), ADMP-1, KIAA0688 protein
Domain architecture
MEROPS Classification
Classification Clan MA >> Subclan MA(M) >> Family M12 >> Subfamily B >> M12.221
HolotypeADAMTS4 peptidase (Homo sapiens), Uniprot accession O75173 (peptidase unit: 213-428), MERNUM MER0005101
History Identifier created: MEROPS 3.21 (12 May 1999)
Activity
Catalytic typeMetallo
PeplistIncluded in the Peplist with identifier PL00196
NC-IUBMBSubclass 3.4 (Peptidases) >> Sub-subclass 3.4.24 (Metalloendopeptidases) >> Peptidase 3.4.24.82
EnzymologyBRENDA database
Proteolytic eventsCutDB database (53 cleavages)
Activity statushuman: active (Hughes et al., 2004)
mouse: active (by similarity)
KnockoutMice in which the ADAMTS4 gene was distrupted exhibited no gross or histologic abnormalities, and indicate that, in the mouse, ADAMTS-4 is not the primary enzyme responsible for aggrecan degradation at the TEGE(373-374)ARGS site Glasson et al., 2004.
Pharmaceutical relevanceLike ADAM-TS5, ADAM-TS4 is a potential drug target for the treatment of osteoarthritis (Malfait et al., 2002).
Other databases WIKIPEDIAhttp://en.wikipedia.org/wiki/ADAMTS4
Cleavage site specificity Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage pattern-/-/-/eScissile bond-/r/g/- (based on 59 cleavages)
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Specificity matrix
 
Amino acid P4 P3 P2 P1 P1' P2' P3' P4'
Gly 5 2 2 4 5 6 15 7
Pro 6 8 1 1 0 3 0 0
Ala 2 6 4 1 8 1 3 2
Val 5 2 1 3 2 1 0 7
Leu 8 8 12 6 6 9 2 4
Ile 4 1 1 1 0 1 0 2
Met 0 1 4 3 2 0 1 3
Phe 3 5 2 0 11 2 1 3
Tyr 3 4 3 6 1 2 2 1
Trp 0 0 1 0 0 0 0 0
Ser 6 3 2 1 3 2 4 4
Thr 5 1 1 3 1 1 0 3
Cys 0 0 0 0 1 1 0 0
Asn 1 4 2 2 3 3 7 4
Gln 1 0 9 1 2 4 4 4
Asp 1 4 1 2 6 1 1 1
Glu 4 7 6 20 2 1 6 4
Lys 3 0 3 2 1 4 5 4
Arg 2 3 3 2 4 16 6 3
His 0 0 1 1 1 1 2 3
Human genetics
Gene symbol Locus Megabases Ensembl Entrez gene Gene Cards OMIM
ADAMTS4 1q31-q32 ENSG00000158859 9507 ADAMTS4 603876
Mouse genetics
Gene symbol Position Megabases Ensembl Entrez gene MGI
Adamts4 1:H3 ENSMUSG00000006403 240913 MGI:1339949