Summary for peptidase M23.002: staphylolysin

Summary Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates

 

Names
MEROPS Namestaphylolysin
Other namesLasA protein (Pseudomonas aeruginosa), pseudoalterin (Pseudoalteromonas sp.), staphylolytic endopeptidase
Domain architecture
MEROPS Classification
Classification Clan MO >> Subclan (none) >> Family M23 >> Subfamily A >> M23.002
Holotypestaphylolysin (Pseudomonas aeruginosa), Uniprot accession P14789 (peptidase unit: 190-369), MERNUM MER0001282
History Identifier created: Handbook of Proteolytic Enzymes (1998) Academic Press, London.
Activity
Catalytic typeMetallo
PeplistIncluded in the Peplist with identifier PL00234
NC-IUBMBNot yet included in IUBMB recommendations.
Proteolytic eventsCutDB database (1 cleavage)
PhysiologyCauses lysis of Staphylococcus aureus cells.
Pharmaceutical relevanceStaphylolysin has been found to be effective in the treatment of experimental S. aureus keratitis in rabbits, and suggested to have potential for the treatment of disease in humans (Barequet et al., 2004).
Cleavage site specificity Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage pattern-/-/G/GScissile bondGaf/-/-/- (based on 12 cleavages)
weblogo
Specificity matrix
 
Amino acid P4 P3 P2 P1 P1' P2' P3' P4'
Gly 0 0 9 12 5 0 0 0
Pro 0 0 0 0 0 0 0 0
Ala 0 0 0 0 4 2 0 1
Val 0 0 0 0 0 0 0 1
Leu 0 3 0 0 0 0 0 0
Ile 0 0 0 0 0 0 0 0
Met 0 0 0 0 0 0 0 0
Phe 0 0 0 0 2 1 0 0
Tyr 0 0 0 0 0 0 0 0
Trp 0 0 0 0 0 0 0 0
Ser 0 0 0 0 0 0 0 0
Thr 0 0 0 0 0 0 0 0
Cys 0 0 0 0 0 0 0 0
Asn 0 0 0 0 0 0 0 0
Gln 0 0 0 0 0 0 2 0
Asp 0 0 0 0 0 0 0 0
Glu 0 0 0 0 0 0 0 0
Lys 0 0 0 0 0 1 0 0
Arg 0 0 0 0 0 0 0 0
His 2 0 0 0 0 0 0 0