Summary for peptidase M27.002: bontoxilysin

Summary Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates Inhibitors Pharma

 

Names
MEROPS Namebontoxilysin
Other namesBoNT/A, BoNT/B, BoNT/C, BoNT/D, BoNT/E, BoNT/F, BoNT/G, BoNT/G, botox A, botulinum neurotoxin type A, botulinum neurotoxin type B, botulinum neurotoxin type C, botulinum neurotoxin type D, botulinum neurotoxin type E, botulinum neurotoxin type F, botulinum neurotoxin type G, botulinum neurotoxin type H, botulinum toxin, Dysport, myobloc
Domain architecture
MEROPS Classification
Classification Clan MA >> Subclan MA(E) >> Family M27 >> Subfamily (none) >> M27.002
Holotypebontoxilysin (Clostridium botulinum), Uniprot accession P10844 (peptidase unit: 118-450), MERNUM MER0001176
History Identifier created: Handbook of Proteolytic Enzymes (1998) Academic Press, London.
Activity
Catalytic typeMetallo
PeplistIncluded in the Peplist with identifier PL00244
NC-IUBMBSubclass 3.4 (Peptidases) >> Sub-subclass 3.4.24 (Metalloendopeptidases) >> Peptidase 3.4.24.69
EnzymologyBRENDA database
Proteolytic eventsCutDB database (8 cleavages)
Pharmaceutical relevanceAs Botox, has therapeutic use for local paralysis of neuromuscular function, as in strabismus (Naumann & Jankovic, 2004).
Cleavage site specificity Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage patterne/a/dsq/qrkScissile bonda/-/etk/k (based on 10 cleavages)
weblogo
Specificity matrix
 
Amino acid P4 P3 P2 P1 P1' P2' P3' P4'
Gly 2 0 0 0 0 0 0 0
Pro 0 0 0 0 0 0 0 0
Ala 1 3 0 1 3 1 0 0
Val 0 0 0 0 0 1 0 0
Leu 0 0 0 0 1 1 1 1
Ile 0 2 0 0 2 0 0 0
Met 0 0 0 0 0 2 0 1
Phe 0 0 0 0 2 0 0 0
Tyr 0 0 0 0 0 0 0 1
Trp 0 0 0 0 0 0 0 0
Ser 0 0 3 0 0 1 1 2
Thr 0 2 0 0 0 1 3 0
Cys 0 0 0 0 0 0 0 0
Asn 0 1 1 0 0 0 0 0
Gln 2 0 2 4 0 0 0 0
Asp 1 1 3 0 0 0 0 0
Glu 3 0 0 0 0 2 3 1
Lys 0 0 1 2 1 1 2 4
Arg 1 1 0 3 1 0 0 0
His 0 0 0 0 0 0 0 0