Summary for peptidase M35.002: deuterolysin

Summary Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates Inhibitors

 

Names
MEROPS Namedeuterolysin
Other namesacid metalloproteinase, neutral proteinase II, NpII g.p. (Aspergillus oryzae), Mep20 g.p. (Aspergillus fumigatus), Penicillium roqueforti protease II, PepJ peptidase (Emericella nidulans)
Domain architecture
MEROPS Classification
Classification Clan MA >> Subclan MA(D) >> Family M35 >> Subfamily (none) >> M35.002
Holotypedeuterolysin (Aspergillus flavus), Uniprot accession P46073 (peptidase unit: 194-381), MERNUM MER0001394
History Identifier created: Handbook of Proteolytic Enzymes (1998) Academic Press, London.
Activity
Catalytic typeMetallo
PeplistIncluded in the Peplist with identifier PL00251
NC-IUBMBSubclass 3.4 (Peptidases) >> Sub-subclass 3.4.24 (Metalloendopeptidases) >> Peptidase 3.4.24.39
EnzymologyBRENDA database
BiotechnologyTaste-forming factor in soy sauce.
Cleavage site specificity Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage patternhgl/vfl/lv/-Scissile bondl/l/l/gv (based on 22 cleavages)
weblogo
Specificity matrix
 
Amino acid P4 P3 P2 P1 P1' P2' P3' P4'
Gly 3 2 1 2 1 0 0 4
Pro 0 0 0 0 0 2 0 0
Ala 0 2 0 2 2 1 1 3
Val 0 5 4 1 3 3 1 4
Leu 3 4 4 1 5 5 7 3
Ile 0 0 0 0 0 0 0 0
Met 0 0 0 0 0 0 0 0
Phe 2 4 2 2 0 1 1 0
Tyr 0 0 0 4 0 2 2 2
Trp 0 0 0 0 0 0 0 0
Ser 1 1 1 1 2 0 0 0
Thr 0 0 0 0 2 0 0 0
Cys 0 0 0 0 0 0 0 0
Asn 0 0 2 2 0 2 0 0
Gln 0 0 0 2 2 0 2 0
Asp 0 0 0 0 0 0 0 0
Glu 2 0 3 2 1 1 1 1
Lys 0 0 0 1 0 0 2 0
Arg 0 0 0 1 0 0 0 0
His 4 1 1 1 3 4 0 2