Summary for peptidase S01.136: granzyme B, rodent-type

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Mouse EST Substrates Inhibitors

 

Names
MEROPS Namegranzyme B, rodent-type
Other namesasp-ase, CCP II, CTLA1, CGL-1, cytotoxic cell protease 2, fragmentin, GzmBP (Mus musculus), GzmBW (Mus musculus)
Domain architecture
MEROPS Classification
Classification Clan PA >> Subclan PA(S) >> Family S1 >> Subfamily A >> S01.136
Holotypegranzyme B, rodent-type (Mus musculus), Uniprot accession P04187 (peptidase unit: 21-247), MERNUM MER0000160
History Identifier created: Handbook of Proteolytic Enzymes (1998) Academic Press, London.
Activity
Catalytic typeSerine
NC-IUBMBSubclass 3.4 (Peptidases) >> Sub-subclass 3.4.21 (Serine endopeptidases) >> Peptidase 3.4.21.79
EnzymologyBRENDA database
Proteolytic eventsCutDB database (22 cleavages)
PhysiologyRole in triggering of apoptosis by cytotoxic cell granules, activating caspases.
Other databases TREEFAMhttp://www.treefam.org/family/TF333630
Cleavage site specificity Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage patternv/-/-/DScissile bond-/-/-/- (based on 487 cleavages)
weblogo
Specificity matrix
 
Amino acid P4 P3 P2 P1 P1' P2' P3' P4'
Gly 5 60 63 0 34 76 18 26
Pro 40 1 76 1 0 3 14 16
Ala 36 40 51 5 72 52 26 33
Val 101 26 60 0 31 15 54 42
Leu 77 47 39 5 61 92 35 46
Ile 73 8 5 0 21 7 38 31
Met 19 10 12 2 7 6 6 10
Phe 21 9 17 0 14 17 10 20
Tyr 6 7 5 2 5 15 26 4
Trp 8 1 1 0 1 0 0 0
Ser 12 34 32 4 74 48 35 33
Thr 11 23 18 2 47 22 37 27
Cys 6 9 0 0 1 1 2 0
Asn 4 20 21 10 12 8 11 7
Gln 11 14 25 0 19 15 12 23
Asp 5 54 10 402 16 42 31 66
Glu 32 73 36 51 34 48 68 75
Lys 8 12 10 1 31 12 58 21
Arg 0 11 0 1 0 1 0 1
His 5 23 2 1 1 1 0 0
Specificity from combinatorial peptides
 
Organism comment P4 P3 P2 P1 P1' P2' P3' P4' optimal substrate fluorophore or acceptor-donor pair Reference
Rattus norvegicus wild type I/V E P/n/A D - - - - IEPD AMC Ruggles et al., 2004
Rattus norvegicus recombinant nd nd nd D - - - - D ACC Harris et al., 2000
Mouse genetics
Gene symbol Position Megabases Ensembl Entrez gene MGI
Gzmb 14:C1 ENSMUSG00000015437 14939 MGI:109267