Summary for peptidase S01.151: trypsin 1

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Mouse EST Substrates Inhibitors Pharma

 

Names
MEROPS Nametrypsin 1
Other namespseudotrypsin, psi-trypsin, trypsin 7 (Mus musculus), trypsin III (cationic) (Rattus norvegicus), trypsin 1 (tetrapod, non-Homo sapiens)
Domain architecture
MEROPS Classification
Classification Clan PA >> Subclan PA(S) >> Family S1 >> Subfamily A >> S01.151
Holotypetrypsin 1 (Bos taurus), Uniprot accession P00760 (peptidase unit: 24-246), MERNUM MER0000024
History Identifier created: Handbook of Proteolytic Enzymes (1998) Academic Press, London.
Activity
Catalytic typeSerine
PeplistIncluded in the Peplist with identifier PL00281
NC-IUBMBSubclass 3.4 (Peptidases) >> Sub-subclass 3.4.21 (Serine endopeptidases) >> Peptidase 3.4.21.4
EnzymologyBRENDA database
Proteolytic eventsCutDB database (29 cleavages)
PhysiologyMajor role in intestinal digestion of food proteins in mammals, and also in the activation of zymogens of other digestive peptidases.
Pathways KEGGInfluenza A
KEGGNeuroactive ligand-receptor interaction
KEGGPancreatic secretion
KEGGProtein digestion and absorption
Other databases WIKIPEDIAhttp://en.wikipedia.org/wiki/Trypsin_1
Cleavage site specificity Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage pattern-/-/-/KRScissile bond-/-/-/- (based on 13770 cleavages)
weblogo
Specificity matrix
 
Amino acid P4 P3 P2 P1 P1' P2' P3' P4'
Gly 864 985 1098 0 908 983 976 841
Pro 873 667 842 0 14 545 845 791
Ala 1193 1191 1113 0 1301 1203 1146 1051
Val 845 1023 879 0 1111 1077 961 949
Leu 1327 1636 1614 1 1348 1756 1276 1317
Ile 773 857 795 0 946 967 778 830
Met 277 314 292 0 348 333 240 260
Phe 457 602 579 2 500 601 638 500
Tyr 320 417 385 5 432 420 356 293
Trp 104 111 115 1 98 124 94 142
Ser 840 889 901 0 901 829 909 888
Thr 707 749 686 0 938 777 776 820
Cys 156 164 180 1 158 186 226 204
Asn 629 556 596 0 602 457 481 467
Gln 702 575 663 0 586 515 619 688
Asp 1069 775 575 1 851 734 829 930
Glu 1375 1051 1183 3 1189 751 1243 1387
Lys 554 612 743 8200 820 748 662 627
Arg 375 293 303 5555 469 502 437 438
His 249 259 199 1 212 211 197 233
Specificity from combinatorial peptides
 
Organism comment P4 P3 P2 P1 P1' P2' P3' P4' optimal substrate fluorophore or acceptor-donor pair Reference
Sus scrofa recombinant broad broad broad R - - - - bbbR AMC Furlong et al., 2002
Homo sapiens recombinant nd nd nd R - - - - R ACC Harris et al., 2000
Bos taurus wild type - L P R H L P - xLPR+HLPx Abz-Dnp Alves et al., 2007
Homo sapiens recombinant Q K G R - - - - QKGR+xxxx Abz-Tyr(NO2) Janssen et al., 2004
Homo sapiens recombinant - M/K/Q R/K - R/S V/I/R G/P/S/D - xMRx+RVGx Abz-EDDnp Bianchini et al., 2002
Bos taurus wild type - K A/broad K - - - - xKAK+xxxx ACC-Dabcyl Sun et al., 2007
Mouse genetics
Gene symbol Position Megabases Ensembl Entrez gene MGI
2210010C04Rik 6:B1 ENSMUSG00000029882 67373 MGI:1914623