Summary for peptidase S01.251: kallikrein-related peptidase 4

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Human EST Substrates Inhibitors Pharma

 

Names
MEROPS Namekallikrein-related peptidase 4
Other namesenamel matrix serine proteinase 1, EMSP1, kallikrein hK4, kallikrein 4 (Homo sapiens), kallikrein-like 1 protein (Homo sapiens), KLK4 g.p. (Homo sapiens), Klk4 g.p. (Mus musculus), prostase, PRSS17 [obs.] g.p. (Homo sapiens), serine protease 17 [obs.]
Domain architecture
MEROPS Classification
Classification Clan PA >> Subclan PA(S) >> Family S1 >> Subfamily A >> S01.251
Holotypekallikrein-related peptidase 4 (Homo sapiens), Uniprot accession Q9Y5K2 (peptidase unit: 31-254), MERNUM MER0005266
History Identifier created: MEROPS 3.02 (25 June 1998)
Activity
Catalytic typeSerine
PeplistIncluded in the Peplist with identifier PL00326
NC-IUBMBNot yet included in IUBMB recommendations.
Proteolytic eventsCutDB database (10 cleavages)
Activity statushuman: active (Simmer, 2004)
mouse: active (by similarity) (Hu et al., 2000)
PhysiologyMutation in prostase causes autosomal recessive hypomaturation amelogenesis imperfecta (Hart et al., 2004).
Pharmaceutical relevanceProposed for use in vaccine strategies against prostate cancer (Hural et al., 2002).
Other databases WIKIPEDIAhttp://en.wikipedia.org/wiki/KLK4
Cleavage site specificity Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage patternr/-/-/RScissile bond-/-/-/- (based on 123 cleavages)
weblogo
Specificity matrix
 
Amino acid P4 P3 P2 P1 P1' P2' P3' P4'
Gly 6 4 8 1 12 8 14 27
Pro 9 2 14 0 1 6 10 12
Ala 5 4 6 1 18 13 3 4
Val 7 10 2 0 6 16 5 5
Leu 9 8 6 3 6 17 6 7
Ile 7 4 5 0 10 12 4 5
Met 0 5 2 2 3 1 2 1
Phe 5 4 3 1 3 4 2 3
Tyr 4 5 3 7 1 4 6 2
Trp 2 1 2 0 1 9 1 1
Ser 7 14 16 0 23 4 13 6
Thr 2 7 8 0 3 6 1 7
Cys 0 0 0 0 1 0 1 2
Asn 4 3 3 2 3 3 11 2
Gln 2 17 5 2 4 3 6 6
Asp 5 2 1 0 5 0 12 3
Glu 3 6 2 0 6 2 8 8
Lys 1 11 23 9 2 3 2 6
Arg 34 7 12 92 3 4 4 4
His 0 3 1 3 4 0 3 3
Specificity from combinatorial peptides
 
Organism comment P4 P3 P2 P1 P1' P2' P3' P4' optimal substrate fluorophore or acceptor-donor pair Reference
Homo sapiens recombinant I/V S/broad Q/L/V R/F - - - - ISQR ACC Borgoņo et al., 2007
Homo sapiens recombinant I/broad broad Q/V/L R - - - - IbQR ACC Debela et al., 2006
Human genetics
Gene symbol Locus Megabases Ensembl Entrez gene Gene Cards OMIM
KLK4 19q13.3-q13.4 ENSG00000167749 9622 KLK4 603767
Mouse genetics
Gene symbol Position Megabases Ensembl Entrez gene MGI
Klk4 7:B2 ENSMUSG00000006948 56640 MGI:1861379