Summary for peptidase S07.001: flavivirin

Summary Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates Inhibitors Pharma

 

Names
MEROPS Nameflavivirin
Other namesdengue virus NS3 protease, encephalitis virus proteinase, Langat virus NS3 peptidase, Murray Valley encephalitis virus proteinase, NS3 protein, NS2B/NS3 peptidase, NS2B-3 proteinase, tick-borne encephalitis virus peptidase, St. Louis encephalitis virus peptidase, West Nile flavivirus proteinase, yellow fever virus (flavivirus) protease
Domain architecture
MEROPS Classification
Classification Clan PA >> Subclan PA(S) >> Family S7 >> Subfamily (none) >> S07.001
Holotypeflavivirin (yellow fever virus), Uniprot accession Q9YRV3 (peptidase unit: 1485-1666), MERNUM MER0000290
History Identifier created: Handbook of Proteolytic Enzymes (1998) Academic Press, London.
Activity
Catalytic typeSerine
PeplistIncluded in the Peplist with identifier PL00340
NC-IUBMBSubclass 3.4 (Peptidases) >> Sub-subclass 3.4.21 (Serine endopeptidases) >> Peptidase 3.4.21.91
EnzymologyBRENDA database
Proteolytic eventsCutDB database (5 cleavages)
PhysiologyContributes to processing of viral polyprotein.
Pharmaceutical relevancePotential drug target.
Cleavage site specificity Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage pattern-/gk/KR/RScissile bondGS/g/-/a (based on 21 cleavages)
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Specificity matrix
 
Amino acid P4 P3 P2 P1 P1' P2' P3' P4'
Gly 3 5 1 0 10 5 2 1
Pro 0 1 0 0 0 0 2 0
Ala 2 3 0 0 0 1 3 4
Val 0 1 0 0 0 0 1 2
Leu 3 1 0 0 0 2 0 3
Ile 0 0 0 0 0 1 1 0
Met 0 0 0 0 0 0 0 0
Phe 1 0 0 0 0 0 0 0
Tyr 1 0 0 0 0 0 0 0
Trp 0 0 0 0 0 1 0 0
Ser 1 1 0 0 6 2 2 1
Thr 1 2 0 0 0 2 2 0
Cys 0 0 0 0 0 0 0 0
Asn 2 0 0 0 0 0 0 2
Gln 1 0 0 0 0 1 0 0
Asp 0 0 0 0 0 0 1 0
Glu 1 0 0 0 0 0 1 1
Lys 0 5 10 2 0 0 0 2
Arg 3 1 10 19 0 1 1 0
His 1 0 0 0 0 0 0 0
Specificity from combinatorial peptides
 
Organism comment P4 P3 P2 P1 P1' P2' P3' P4' optimal substrate fluorophore or acceptor-donor pair Reference
Dengue virus 3 recombinant n/L/K K/G/R R R - - - - nKRR ACC Li et al., 2005
dengue virus 4 recombinant - - - - S G/D S G/N xxxx+SGSG AMCA-Dnp Li et al., 2005
dengue virus 3 recombinant - - - - S D/G/P S G xxxx+SDSG AMCA-Dnp Li et al., 2005
dengue virus 2 recombinant - - - - S G S G/N xxxx+SGSG AMCA-Dnp Li et al., 2005
dengue virus 1 recombinant - - - - S D/E S N/D/G xxxx+SDSN AMCA-Dnp Li et al., 2005
Yellow fever virus recombinant - G/E K/R K/R T/S L/A R/I - xGKK+TLRx Dnp-Abz Alves et al., 2007
Yellow fever virus recombinant R R R R/K S A G - RRRR+SAGx Abz-Tyr(NO2) Nigomrattanakit et al., 2006
Dengue virus 1 recombinant n/L K R/T R - - - - nKRR ACC Li et al., 2005
Dengue virus 4 recombinant L/K/n K R R - - - - LKRR ACC Li et al., 2005
Dengue virus 2 recombinant n/L/K K/R R/T R - - - - nKRR ACC Li et al., 2005