Summary for peptidase S08.001: subtilisin Carlsberg

Summary Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates Inhibitors Pharma

 

Names
MEROPS Namesubtilisin Carlsberg
Other namesAlcalase Novo, kerA g.p. (Bacillus licheniformis), Ker BL (Bacillus licheniformis), Ker BP (Bacillus pumilus), keratinase (Bacillus sp.), subtilisin A, subtilisin JB1, subtilopeptidase A, thiolsubtilisin, Thrombovasim
Domain architecture
MEROPS Classification
Classification Clan SB >> Subclan (none) >> Family S8 >> Subfamily A >> S08.001
Holotypesubtilisin Carlsberg (Bacillus licheniformis), Uniprot accession P00780 (peptidase unit: 106-379), MERNUM MER0000309
History Identifier created: Handbook of Proteolytic Enzymes (1998) Academic Press, London.
Activity
Catalytic typeSerine
PeplistIncluded in the Peplist with identifier PL00342
NC-IUBMBSubclass 3.4 (Peptidases) >> Sub-subclass 3.4.21 (Serine endopeptidases) >> Peptidase 3.4.21.62
EnzymologyBRENDA database
BiotechnologyForms of subtilisin are widely used commercially.
Cleavage site specificity Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage pattern-/-/-/-Scissile bondas/-/-/- (based on 43 cleavages)
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Specificity matrix
 
Amino acid P4 P3 P2 P1 P1' P2' P3' P4'
Gly 4 4 4 0 2 1 4 5
Pro 3 0 6 0 0 1 3 4
Ala 5 6 8 6 8 1 4 2
Val 2 1 6 3 5 5 3 1
Leu 2 4 1 8 4 5 2 2
Ile 0 0 1 0 0 2 0 0
Met 1 0 0 1 1 0 1 0
Phe 2 2 1 2 1 1 1 0
Tyr 7 0 1 5 2 1 1 2
Trp 0 0 0 0 0 0 0 0
Ser 4 6 6 5 7 4 5 4
Thr 3 5 2 0 4 4 2 3
Cys 0 0 0 0 0 0 0 1
Asn 1 2 1 1 0 0 1 1
Gln 0 2 1 3 1 1 0 1
Asp 0 1 0 0 0 0 0 2
Glu 2 1 1 2 0 3 0 3
Lys 0 3 0 2 0 0 4 1
Arg 0 1 1 2 0 2 2 0
His 3 4 3 2 2 3 1 2
Specificity from combinatorial peptides
 
Organism comment P4 P3 P2 P1 P1' P2' P3' P4' optimal substrate fluorophore or acceptor-donor pair Reference
Bacillus licheniformis wild type - - - K - - - - xxxK+xxxx ACC-Dabcyl Sun et al., 2007
Bacillus licheniformis wild type V/M/F Q P L D E - - VQPL+DExx Abz-Tyr(NO2) Meldal et al., 1991