Summary for peptidase S09.001: prolyl oligopeptidase

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Human EST Mouse EST Substrates Inhibitors Pharma

 

Names
MEROPS Nameprolyl oligopeptidase
Other namesdpoA g.p. (Dictyostelium sp.), endo-oligopeptidase B, endoproteinase Pro-C, post-proline cleaving enzyme, proline-specific endopeptidase, prolyl endopeptidase
Domain architecture
MEROPS Classification
Classification Clan SC >> Subclan (none) >> Family S9 >> Subfamily A >> S09.001
Holotypeprolyl oligopeptidase (Sus scrofa), Uniprot accession P23687 (peptidase unit: 428-710), MERNUM MER0000392
History Identifier created: Handbook of Proteolytic Enzymes (1998) Academic Press, London.
Activity
Catalytic typeSerine
PeplistIncluded in the Peplist with identifier PL00367
NC-IUBMBSubclass 3.4 (Peptidases) >> Sub-subclass 3.4.21 (Serine endopeptidases) >> Peptidase 3.4.21.26
EnzymologyBRENDA database
Proteolytic eventsCutDB database (9 cleavages)
Activity statushuman: active (Ito et al., 2004)
mouse: active (Odaka et al., 2002)
PhysiologyCytosolic hydrolysis of oligopeptides.
Pharmaceutical relevanceProlyl oligopeptidase attracted interest for possible neurological activities. Inhibitors of the enzyme have been shown to increase concentrations of several neuropeptides in the brain, and to have anti-amnesic activity in rat (Kamei et al., 1992; Li et al., 1996). Accordingly, the enzyme has been regarded as a therapeutic target (e.g. Venalainen et al., 2006).
Other databases TREEFAMhttp://www.treefam.org/family/TF300655
Cleavage site specificity Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage patternp/pq/-/PScissile bondq/Pg/q/pl (based on 59 cleavages)
weblogo
Specificity matrix
 
Amino acid P4 P3 P2 P1 P1' P2' P3' P4'
Gly 8 3 7 0 7 7 4 2
Pro 9 15 5 50 1 22 3 14
Ala 0 4 1 5 1 0 1 2
Val 0 2 0 1 1 3 1 2
Leu 7 5 7 0 3 3 0 7
Ile 0 2 0 0 0 1 0 1
Met 0 0 0 0 0 1 0 0
Phe 2 5 2 0 6 1 3 1
Tyr 3 0 7 0 7 0 1 2
Trp 2 0 1 0 0 0 0 1
Ser 1 0 3 0 2 0 2 1
Thr 0 0 1 0 1 0 0 0
Cys 0 0 2 0 0 0 0 0
Asn 0 2 3 0 1 0 0 2
Gln 6 8 5 1 15 1 18 0
Asp 2 1 0 0 1 2 0 1
Glu 0 0 0 0 2 0 3 1
Lys 1 0 3 0 1 0 2 0
Arg 3 2 9 0 2 3 0 1
His 1 0 1 0 0 0 1 0
Human genetics
Gene symbol Locus Megabases Ensembl Entrez gene Gene Cards OMIM
PREP 6q22 ENSG00000085377 5550 PREP 600400
Mouse genetics
Gene symbol Position Megabases Ensembl Entrez gene MGI
Prep 10:B2-B3 ENSMUSG00000048634 19072 MGI:1270863