Summary for peptidase S09.007: fibroblast activation protein alpha subunit

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Human EST Mouse EST Substrates Inhibitors Pharma

 

Names
MEROPS Namefibroblast activation protein alpha subunit
Other namesantiplasmin-cleaving enzyme, APCE, FAP alpha, seprase
Domain architecture
MEROPS Classification
Classification Clan SC >> Subclan (none) >> Family S9 >> Subfamily B >> S09.007
Holotypefibroblast activation protein alpha subunit (Homo sapiens), Uniprot accession Q12884 (peptidase unit: 498-758), MERNUM MER0000399
History Identifier created: Handbook of Proteolytic Enzymes (1998) Academic Press, London.
Activity
Catalytic typeSerine
PeplistIncluded in the Peplist with identifier PL00370
NC-IUBMBSubclass 3.4 (Peptidases) >> Sub-subclass () >> Peptidase 3.4.21.B28
EnzymologyBRENDA database
Activity statushuman: active (Park & Rettig, 2004)
mouse: active (Niedermeyer et al., 1998)
PreparationRecombinant FAP is active when made using baculovirus (Keane et al., 2011; Aertgeerts et al., 2005; Wang et al., 2005; Jones et al., 2003).
SpecificityFAP is a dipeptidyl peptidase with a preference for Pro in P1. It also acts as an post-proline cleaving endopeptidase (Lee et al., 2006; Park et al., 1999).
Substrate commentsSubstrates include: alpha 2-antiplasmin (Lee et al., 2006); certain neuropeptides (Keane et al., 2011); denatured collagen I (Park et al., 1999; Levy et al., 1999); and the synthetic substrates benzoyloxycarbonyl (Z)-Gly-Pro, H-Ala-Pro. Weak activity on H-Gly-Pro. Succinyl-Gly-Pro and acyl-Gly-Pro are not cleaved.
Structure1Z68 (Aertgeerts et al., 2005)
LocationFAP is a cell surface type 2 glycoprotein (Scanlan et al., 1994). A soluble form exists in blood (Lee et al., 2006).
RNA splicingnone identified.
KnockoutGene knockout is healthy with normal litters and body weight (Niedermeyer et al., 2000).
Pharmaceutical relevanceEndopeptidase activity is suggested to contribute to degradation of extracellular matrix proteins in malignant invasion.
Contributing authorsMark D. Gorrell, Centenary Institute, Sydney Medical School, University of Sydney, NSW 2006, Australia
Other databases TREEFAMhttp://www.treefam.org/family/TF313309
Human genetics
Gene symbol Locus Megabases Ensembl Entrez gene Gene Cards OMIM
FAP 2q23 ENSG00000078098 2191 FAP 600403
Mouse genetics
Gene symbol Position Megabases Ensembl Entrez gene MGI
Fap 2:C1.3 ENSMUSG00000000392 14089 MGI:109608