Summary for peptidase S14.001: peptidase Clp (type 1)

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates Inhibitors

 

Names
MEROPS Namepeptidase Clp (type 1)
Other namescaseinolytic protease, ClpP, endopeptidase Ti, LmCP2 (Listeria monocytogenes), protease Ti
Domain architecture
MEROPS Classification
Classification Clan SK >> Subclan (none) >> Family S14 >> Subfamily (none) >> S14.001
Holotypepeptidase Clp (type 1) (Escherichia coli), Uniprot accession Q1RF98 (peptidase unit: 1-207), MERNUM MER0000474
History Identifier created: Handbook of Proteolytic Enzymes (1998) Academic Press, London.
Activity
Catalytic typeSerine
PeplistIncluded in the Peplist with identifier PL00382
NC-IUBMBSubclass 3.4 (Peptidases) >> Sub-subclass 3.4.21 (Serine endopeptidases) >> Peptidase 3.4.21.92
EnzymologyBRENDA database
StructureProteolytically active Clp peptidase is a complex of the catalytic subunit, ClpP, with another subunit, commonly ClpA. ClpA has protein-unfolding and ATPase activities, and makes an essential contribution to proteolysis by the complex, which is termed ClpAP. ClpA can be replaced by other subunits with similar activites such as ClpX (Maurizi, 2004). The activities of ClpAP and ClpXP are not identical. ClpP subunits are arranged in seven-membered rings, and the native protein is formed of two such rings. In the presence of ATP, ClpA and ClpX assemble into six-membered rings that have a high affinity for the rings of ClpP.
PhysiologyThought to contribute to elimination of damaged proteins in heat shock.
Cleavage site specificity Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage patternA/p/-/mlScissile bonday/L/V/P (based on 15 cleavages)
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Specificity matrix
 
Amino acid P4 P3 P2 P1 P1' P2' P3' P4'
Gly 1 0 0 1 0 1 1 0
Pro 0 6 0 0 0 0 0 7
Ala 8 0 1 1 7 0 0 0
Val 1 1 1 0 1 0 8 1
Leu 1 2 1 4 1 8 2 0
Ile 0 1 1 0 0 0 0 0
Met 0 0 0 7 0 0 0 0
Phe 0 1 0 0 1 1 0 0
Tyr 0 0 2 1 2 1 1 0
Trp 0 0 1 0 0 1 0 0
Ser 0 0 1 0 0 0 1 0
Thr 0 0 0 0 0 0 0 1
Cys 0 0 0 0 0 0 0 0
Asn 1 0 0 1 0 0 0 0
Gln 0 1 0 0 1 0 0 0
Asp 0 0 0 0 0 0 0 0
Glu 0 2 2 0 0 0 0 1
Lys 0 0 0 0 0 0 0 0
Arg 0 0 1 0 0 0 0 0
His 0 0 4 0 0 1 0 1