Summary for peptidase C01.001: papain

Summary Alignment Sequences Sequence features Distribution Structure Literature Substrates Inhibitors Pharma

 

Names
MEROPS Namepapain
Other namespapaya peptidase I
Domain architecture
MEROPS Classification
Classification Clan CA >> Subclan (none) >> Family C1 >> Subfamily A >> C01.001
Holotypepapain (Carica papaya), Uniprot accession P00784 (peptidase unit: 134-345), MERNUM MER0000647
History Identifier created: Handbook of Proteolytic Enzymes (1998) Academic Press, London.
Activity
Catalytic typeCysteine
PeplistIncluded in the Peplist with identifier PL00046
NC-IUBMBSubclass 3.4 (Peptidases) >> Sub-subclass 3.4.22 (Cysteine endopeptidases) >> Peptidase 3.4.22.2
EnzymologyBRENDA database
Proteolytic eventsCutDB database (3 cleavages)
BiotechnologyUsed in food processing.
PhysiologyBelieved to confer resistance to pests on the source plants.
Other databases WIKIPEDIAhttp://en.wikipedia.org/wiki/Papain
Cleavage site specificity Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage pattern-/-/l/rScissile bondQ/Q/-/D (based on 87 cleavages)
weblogo
Specificity matrix
 
Amino acid P4 P3 P2 P1 P1' P2' P3' P4'
Gly 3 3 1 6 4 4 4 3
Pro 2 9 2 1 0 4 3 4
Ala 3 3 3 3 7 1 2 1
Val 1 2 6 2 3 1 1 3
Leu 4 7 36 6 4 3 4 2
Ile 1 1 1 1 1 2 0 1
Met 0 1 3 4 1 0 0 0
Phe 1 2 8 10 2 2 2 1
Tyr 1 1 4 3 2 2 2 2
Trp 0 0 1 1 0 0 0 0
Ser 1 2 1 2 3 2 1 1
Thr 1 0 5 4 2 2 0 1
Cys 1 0 1 2 0 1 1 0
Asn 1 0 1 3 1 1 0 1
Gln 0 1 1 1 46 42 0 0
Asp 1 1 1 2 1 0 0 46
Glu 10 2 3 2 2 10 4 3
Lys 0 1 2 1 1 0 3 9
Arg 2 1 2 28 0 3 9 1
His 2 1 2 3 1 2 1 1
Specificity from combinatorial peptides
 
Organism comment P4 P3 P2 P1 P1' P2' P3' P4' optimal substrate fluorophore or acceptor-donor pair Reference
Carica papaya wild type P P V R/Q/A A/S/T S/R G/K/P/R P/Q/N PPVR+ASGP Abz-Tyr(NO2) St Hilaire et al., 1999
Carica papaya wild type P/G/H/broad P/R V R/K - - - - PPVR ACC Choe et al., 2006
Carica papaya wild type - - - K - - - - xxxK+xxxx ACC-Dabcyl Sun et al., 2007
Carica papaya wild type - - - Iaf/R - - - - xxxIaf+xxxx Abz-EDDnp Alves et al., 2001
Carica papaya wild type - - - - L/A/S/F - - - xxxx+Lxxx Dansyl-Trp Ménard et al., 1993
Carica papaya wild type - - - - L - - - xxxx+Lxxx Abz-EDDnp Melo et al., 2001
Carica papaya wild type - - - G - - - - xxxG+xxxx Abz-EDDnp Del Nery et al., 2000