Summary for peptidase M10.014: membrane-type matrix metallopeptidase-1

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Human EST Mouse EST Substrates Inhibitors Pharma

 

Names
MEROPS Namemembrane-type matrix metallopeptidase-1
Other namesmatrix metalloproteinase 14, membrane-type matrix metalloproteinase 1, MMP-14, matrix metalloproteinase, membrane-type 1, MT1-MMP, MTMMP-1
Domain architecture
MEROPS Classification
Classification Clan MA >> Subclan MA(M) >> Family M10 >> Subfamily A >> M10.014
Holotypemembrane-type matrix metallopeptidase-1 (Homo sapiens), Uniprot accession P50281 (peptidase unit: 108-301), MERNUM MER0001077
History Identifier created: Handbook of Proteolytic Enzymes (1998) Academic Press, London.
Activity
Catalytic typeMetallo
PeplistIncluded in the Peplist with identifier PL00165
NC-IUBMBSubclass 3.4 (Peptidases) >> Sub-subclass 3.4.24 (Metalloendopeptidases) >> Peptidase 3.4.24.80
EnzymologyBRENDA database
Proteolytic eventsCutDB database (110 cleavages)
Activity statushuman: active (Itoh & Seiki, 2004)
mouse: active (Kinoh et al., 1996)
PhysiologyRole in activation of proform of gelatinase A. Developmentally regulated.
KnockoutMice deficient in membrane-type matrix metalloproteinase 1 display severe defects in skeletal development including dwarfism, osteopenia, and craniofacial abnormalities and delayed tooth eruption. Death occurs in these mice by about 3 weeks of age (Bartlett et al., 2003). There are also defects in lung development (Irie et al., 2005). Mice lacking gelatinase A as well as membrane-type matrix metalloproteinase 1 die immediately after birth (Oh et al., 2004).
Pharmaceutical relevanceBelieved to be important in tumor cell invasion (Iida et al., 2004).
Pathways KEGGGnRH signaling pathway
KEGGTNF signaling pathway
Other databases WIKIPEDIAhttp://en.wikipedia.org/wiki/MMP14
Cleavage site specificity Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage pattern-/p/-/-Scissile bondl/-/-/- (based on 132 cleavages)
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Specificity matrix
 
Amino acid P4 P3 P2 P1 P1' P2' P3' P4'
Gly 14 7 12 27 7 3 12 10
Pro 5 34 6 8 1 6 3 10
Ala 8 13 19 16 3 9 12 11
Val 8 11 8 0 6 13 6 4
Leu 16 16 11 2 46 6 13 10
Ile 3 4 3 0 12 2 1 3
Met 1 2 3 2 7 3 1 0
Phe 2 3 3 1 4 7 4 6
Tyr 0 3 3 4 8 4 1 5
Trp 0 1 0 1 1 2 0 1
Ser 9 16 12 11 9 12 12 9
Thr 5 3 5 3 5 5 12 7
Cys 1 1 1 0 0 0 1 0
Asn 6 2 4 14 6 7 7 8
Gln 10 5 16 6 6 11 8 8
Asp 14 2 3 6 4 4 4 4
Glu 7 2 8 7 1 12 13 12
Lys 7 2 5 8 1 9 7 6
Arg 10 3 6 12 3 13 10 12
His 5 2 3 4 0 4 5 4
Human genetics
Gene symbol Locus Megabases Ensembl Entrez gene Gene Cards OMIM
MMP14 14q11-q12 ENSG00000157227 4323 MMP14 600754
Mouse genetics
Gene symbol Position Megabases Ensembl Entrez gene MGI
Mmp14 14:C1 ENSMUSG00000000957 17387 MGI:101900